IGEM:Harvard/2006/DNA nanostructures/Notebook/2006-9-12

Designed and Ordered Aptamer Oligos for c5.0

• Since the streptavidin-biotinylation protection assay showed success, and since our original plan was to work with a more in vivo useful biological pairing (ie. thrombin to thrombin-aptamer), I thought it would be a good idea to order the appropriate thrombin-aptamer-containing oligos for a protection assay.

• We only need 10 new oligos, without biotinylation obviously, so they're not too expensive - better to have them on hand when we need them than have to wait for them later.

• Used the convention from c4.0 of: original (split) sequence (taken from the sequences of the biotinylated revised oligos c5.0.8r and c5.0.9r), 3Ts, followed by the thrombin-aptamer sequence ggttggtgtggttgg.

• See Notebook on 9.12 for the sequences ordered, and what oligos from older orders will be needed to add to them.