IGEM:Harvard/2006/Folding buffers

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< IGEM:Harvard | 2006(Difference between revisions)
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Current revision (12:59, 14 August 2006) (view source)
 
Line 16: Line 16:
| 300||<b>30</b>||200 {{uL}}||40 {{ul}}||120 {{ul}}||40 {{ul}}
| 300||<b>30</b>||200 {{uL}}||40 {{ul}}||120 {{ul}}||40 {{ul}}
|}
|}
 +
 +
For 0.1% SDS (in 1x solution), add 20 {{ul}} 20% SDS.
 +
For 0.01% SDS (in 1x solution), add 2 {{ul}} 20% SDS.

Current revision

  • for a desired final MgCl2 concentration, mix the reagents in a given row
  • yields 400 μL of 10x buffer
Final [MgCl2] in 10x Final [MgCl2] in 1x HEPES (1 M, pH 7.5) NaCl (5 M) MgCl2 (1 M) H2O
10010200 μL40 μL40 μL120 μL
20020200 μL40 μL80 μL80 μL
30030200 μL40 μL120 μL40 μL

For 0.1% SDS (in 1x solution), add 20 μL 20% SDS. For 0.01% SDS (in 1x solution), add 2 μL 20% SDS.

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