IGEM:Harvard/2006/Lab Intro

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Parts:
Parts:
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* amp resistance
* amp resistance
-
Digest R0010 - SpeI + PstI (prefix)
+
*Digest R0010 - SpeI + PstI (prefix)
-
Digest E0241 - XbaI + PstI (Suffix)
+
*Digest E0241 - XbaI + PstI (Suffix)
Gel extraction -> Ligation -> Transformation -> Miniprep -> Sequencing
Gel extraction -> Ligation -> Transformation -> Miniprep -> Sequencing

Revision as of 12:50, 6 June 2006

Parts:

R0010 - LacI promoter

  • Plate 1, Well 7K
  • amp resistance

E0241 - GFP reporter

  • Plate 2, Well 15L
  • amp resistance

E7104 - GFP reporter behind T7 promoter

  • Plate 2, Well 13F
  • amp resistance
  • Digest R0010 - SpeI + PstI (prefix)
  • Digest E0241 - XbaI + PstI (Suffix)

Gel extraction -> Ligation -> Transformation -> Miniprep -> Sequencing

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