IGEM:Harvard/2006/Lab Intro

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Line 4: Line 4:
* Plate 1, Well 7K
* Plate 1, Well 7K
* amp resistance
* amp resistance
 +
* Hydrated yellow
E0241 - GFP reporter
E0241 - GFP reporter
* Plate 2, Well 15L
* Plate 2, Well 15L
* amp resistance
* amp resistance
 +
* Hydrated red
E7104 - GFP reporter behind T7 promoter
E7104 - GFP reporter behind T7 promoter
* Plate 2, Well 13F
* Plate 2, Well 13F
* amp resistance
* amp resistance
 +
* Hydrated red
*Digest R0010 - SpeI + PstI (prefix)
*Digest R0010 - SpeI + PstI (prefix)

Revision as of 14:56, 6 June 2006

Parts:

R0010 - LacI promoter

  • Plate 1, Well 7K
  • amp resistance
  • Hydrated yellow

E0241 - GFP reporter

  • Plate 2, Well 15L
  • amp resistance
  • Hydrated red

E7104 - GFP reporter behind T7 promoter

  • Plate 2, Well 13F
  • amp resistance
  • Hydrated red
  • Digest R0010 - SpeI + PstI (prefix)
  • Digest E0241 - XbaI + PstI (Suffix)

Gel extraction -> Ligation -> Transformation -> Miniprep -> Sequencing

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