IGEM:Harvard/2006/Lab Intro: Difference between revisions
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* Plate 1, Well 7K | * Plate 1, Well 7K | ||
* amp resistance | * amp resistance | ||
* Hydrated yellow | |||
E0241 - GFP reporter | E0241 - GFP reporter | ||
* Plate 2, Well 15L | * Plate 2, Well 15L | ||
* amp resistance | * amp resistance | ||
* Hydrated red | |||
E7104 - GFP reporter behind T7 promoter | E7104 - GFP reporter behind T7 promoter | ||
* Plate 2, Well 13F | * Plate 2, Well 13F | ||
* amp resistance | * amp resistance | ||
* Hydrated red | |||
*Digest R0010 - SpeI + PstI (prefix) | *Digest R0010 - SpeI + PstI (prefix) | ||
Revision as of 11:56, 6 June 2006
Parts:
R0010 - LacI promoter
- Plate 1, Well 7K
- amp resistance
- Hydrated yellow
E0241 - GFP reporter
- Plate 2, Well 15L
- amp resistance
- Hydrated red
E7104 - GFP reporter behind T7 promoter
- Plate 2, Well 13F
- amp resistance
- Hydrated red
- Digest R0010 - SpeI + PstI (prefix)
- Digest E0241 - XbaI + PstI (Suffix)
Gel extraction -> Ligation -> Transformation -> Miniprep -> Sequencing
