IGEM:Harvard/2006/Lab Intro

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Gel extraction -> Ligation -> Transformation -> Miniprep -> Sequencing
Gel extraction -> Ligation -> Transformation -> Miniprep -> Sequencing
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[http://parts.mit.edu/wiki/index.php/Biobrick_delivery Biobrick Delivery]
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[[Transforming_chemically_competent_cells]]

Revision as of 15:14, 6 June 2006

Parts:

R0010 - LacI promoter

  • Plate 1, Well 7K
  • amp resistance
  • Hydrated yellow

E0241 - GFP reporter

  • Plate 2, Well 15L
  • amp resistance
  • Hydrated red

E7104 - GFP reporter behind T7 promoter

  • Plate 2, Well 13F
  • amp resistance
  • Hydrated red
  • Digest R0010 - SpeI + PstI (prefix)
  • Digest E0241 - XbaI + PstI (Suffix)

Gel extraction -> Ligation -> Transformation -> Miniprep -> Sequencing

Biobrick Delivery

Transforming_chemically_competent_cells

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