IGEM:Harvard/2006/Lab Intro: Difference between revisions
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Gel extraction -> Ligation -> Transformation -> Miniprep -> Sequencing | Gel extraction -> Ligation -> Transformation -> Miniprep -> Sequencing | ||
[http://parts.mit.edu/wiki/index.php/Biobrick_delivery Biobrick Delivery] | |||
[[Transforming_chemically_competent_cells]] | |||
Revision as of 12:14, 6 June 2006
Parts:
R0010 - LacI promoter
- Plate 1, Well 7K
- amp resistance
- Hydrated yellow
E0241 - GFP reporter
- Plate 2, Well 15L
- amp resistance
- Hydrated red
E7104 - GFP reporter behind T7 promoter
- Plate 2, Well 13F
- amp resistance
- Hydrated red
- Digest R0010 - SpeI + PstI (prefix)
- Digest E0241 - XbaI + PstI (Suffix)
Gel extraction -> Ligation -> Transformation -> Miniprep -> Sequencing
