IGEM:Harvard/2006/NES Notebook: Difference between revisions
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==To Order== | |||
*Spe1 | |||
*Competent Bacteria | |||
*Small, Medium, Large, X-large Nitrile gloves | |||
==Introductory Materials== | ==Introductory Materials== | ||
[[IGEM:Harvard/2006/Lab_Intro]] | [[IGEM:Harvard/2006/Lab_Intro]] | ||
Revision as of 17:02, 14 June 2006
To Order
- Spe1
- Competent Bacteria
- Small, Medium, Large, X-large Nitrile gloves
Introductory Materials
New Reporters
Due to issues with T7 promoter, the following biobricks were rehydrated 6/14
Constitutive (IPTG-inducable?) GFP
Constitutive (IPTG?) RFP
Constitutive GFP using TetR promoter. No experience; might not work.
Constitutive (IPTG?) YFP. Apparently gives uneven YFP expression
Transformed with 2ul of DNA on 15ul bacteria; twice for each plasmid. Took 3 ul from each incubation of and added into two two mixed SOC cultures. Let's see what happens when LacI GFP, RFP, YFP are co-cultured.
One of each LacI plasmid type, GFP, RFP, YFP, LacI were cultured overnight on plates treated with 10mM IPTG.
