IGEM:Harvard/2006/NES Notebook: Difference between revisions
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One of each LacI plasmid type, GFP, RFP, YFP, LacI were cultured overnight on plates treated with 10mM IPTG. | One of each LacI plasmid type, GFP, RFP, YFP, LacI were cultured overnight on plates treated with 10mM IPTG. | ||
==Microscope Notes== | ==Microscope Notes== | ||
GFP | Overlap between FITC and YGFP blocks prevents differentiation between YFP and GFP. RFP can be differentiated between YFP/GFP very well. | ||
YFP/GFP fades ~3 hours after being taken out of the incubator after overnight incubation. RFP was still strong at +3h. | |||
Revision as of 12:08, 15 June 2006
To Order
- Spe1
- Competent Bacteria -- Ordered by Alain
- Small, Medium, Large, X-large Nitrile gloves
Introductory Materials
New Reporters
Due to issues with T7 promoter, the following biobricks were rehydrated 6/14
Constitutive (IPTG-inducable?) GFP
Constitutive (IPTG?) RFP
Constitutive GFP using TetR promoter. No experience; might not work.
Constitutive (IPTG?) YFP. Apparently gives uneven YFP expression
Transformed with 2ul of DNA on 15ul bacteria; twice for each plasmid. Took 3 ul from each incubation of and added into two two mixed SOC cultures. Let's see what happens when LacI GFP, RFP, YFP are co-cultured.
One of each LacI plasmid type, GFP, RFP, YFP, LacI were cultured overnight on plates treated with 10mM IPTG.
Microscope Notes
Overlap between FITC and YGFP blocks prevents differentiation between YFP and GFP. RFP can be differentiated between YFP/GFP very well.
YFP/GFP fades ~3 hours after being taken out of the incubator after overnight incubation. RFP was still strong at +3h.
