IGEM:Harvard/2006/vlau/Notebook/2006-7-19: Difference between revisions
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===''Pre-Working Stocks''=== | ===''Pre-Working Stocks & Working Stocks''=== | ||
1. Rxn Mixtures | |||
{| {{table}} | {| {{table}} | ||
| align="center" style="background:#f0f0f0;"|'''Description''' | | align="center" style="background:#f0f0f0;"|'''Description''' | ||
| Line 57: | Line 56: | ||
|c4.0.25: bottom lid zipper oligos||box 4_C: row C (7-8)||2 | |c4.0.25: bottom lid zipper oligos||box 4_C: row C (7-8)||2 | ||
|} | |} | ||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Working Stocks''' | |||
| align="center" style="background:#f0f0f0;"|'''Description''' | |||
| align="center" style="background:#f0f0f0;"|'''Pre-Working Stocks''' | |||
| align="center" style="background:#f0f0f0;"|'''Water''' | |||
| align="center" style="background:#f0f0f0;"|'''Total''' | |||
|- | |||
|c4.0A||no latches, no aptamers||1 (77 {{ul}}), 2 (34 {{ul}}), 3 (34 {{ul}}), 4 (4 {{ul}}), 6 (4 {{ul}}), 10 (4 {{ul}}), 15 (2 {{ul}}), 16 (2 {{ul}})||39 {{ul}}||200 {{ul}} | |||
|- | |||
|c4.0B.core||latch design 1, no aptamers||1 (77 {{ul}}), 2 (34 {{ul}}), 3 (34 {{ul}}), 4 (4 {{ul}}), 6 (4 {{ul}})||47 {{ul}}||200 {{ul}} | |||
|- | |||
|c4.0B.latches||latch design 1, no aptamers||8 (4 {{ul}}), 11 (2 {{ul}}), 12 (2 {{ul}})||-||- | |||
|- | |||
|c4.0C.core||latch design 2, no aptamers||1 (77 {{ul}}), 2 (34 {{ul}}), 3 (34 {{ul}}), 4 (4 {{ul}}), 6 (4 {{ul}})||47 {{ul}}||200 {{ul}} | |||
|- | |||
|c4.0C.latches||latch design 2, no aptamers||9 (4 {{ul}}), 13 (2 {{ul}}), 14 (2 {{ul}}), 23 (4 {{ul}}), 24 (2 {{ul}}), 25 (2 {{ul}})||-||- | |||
|} | |||
2. Protocol | |||
- mixed 10 {{ul}} of each 50 {{um}} stock oligo to make pre-working stocks | |||
- mixed listed pre-working stocks to make working stocks where final concentration of each oligo is 250 nM | |||
Revision as of 10:03, 19 July 2006
Pre-Working Stocks & Working Stocks
1. Rxn Mixtures
| Description | Plate Locations | Total |
| c4.0.1: core barrel oligos | box 4_A: row A (1-12), row B (1-12), row C (1-12), row D (1-12), row E (1-12), row F (1-6) | 77 |
| c4.0.2: core top lid oligos | box 4_A: row F (7-12), row G (1-12), row H (1-12); box 4_B: row A (1-3) | 34 |
| c4.0.3: core bottom lid oligos | box 4_B: row A (4-12), row B (1-12), row C (1-12) | 34 |
| c4.0.4: barrel oligos @ outside aptamers -aptamers | box 4_B: row D (1-4) | 4 |
| c4.0.5: barrel oligos @ outside aptamers +aptamers | box 4_B: row D (5-8) | 4 |
| c4.0.6: barrel oligos @ inside aptamers -aptamers | box 4_B: row D (9-12) | 4 |
| c4.0.7: barrel oligos @ inside aptamers +aptamers | box 4_B: row E (1-4) | 4 |
| c4.0.8: two-oligo latch sequences for barrel | box 4_B: row E (5-8) | 4 |
| c4.0.9: four-oligo latch sequences for barrel | box 4_B: row E (9-12) | 4 |
| c4.0.10: barrel oligos @ latches -latches | box 4_B: row F (1-4) | 4 |
| c4.0.11: two-oligo latch sequences for top lid | box 4_B: row F (5-6) | 2 |
| c4.0.12: two-oligo latch sequences for bottom lid | box 4_B: row F (7-8) | 2 |
| c4.0.13: four-oligo latch sequences for top lid | box 4_C: row A (1-2) | 2 |
| c4.0.14: four-oligo latch seequences for bottom lid | box 4_C: row A (3-4) | 2 |
| c4.0.15: top lid oligos @ latches -latches | box 4_C: row A (5-6) | 2 |
| c4.0.16: bottom lid oligos @ latches -latches | box 4_C: row A (7-8) | 2 |
| c4.0.17: two-oligo latch barrel displacement oligos | box 4_C: row A (9-12) | 4 |
| c4.0.18: two-oligo latch top lid displacement oligos | box 4_C: row B (1-2) | 2 |
| c4.0.19: two-oligo latch bottom lid displacement oligos | box 4_C: row B (3-4) | 2 |
| c4.0.20: four-oligo latch barrel displacement oligos | box 4_C: row B (5-8) | 4 |
| c4.0.21: four-oligo latch top lid displacement oligos | box 4_C: row B (9-10) | 2 |
| c4.0.22: four-oligo latch bottom lid displacement oligos | box 4_C: row B (11-12) | 2 |
| c4.0.23: barrel zipper oligos | box 4_C: row C (1-4) | 4 |
| c4.0.24: top lid zipper oligos | box 4_C: row C (5-6) | 2 |
| c4.0.25: bottom lid zipper oligos | box 4_C: row C (7-8) | 2 |
| Working Stocks | Description | Pre-Working Stocks | Water | Total |
| c4.0A | no latches, no aptamers | 1 (77 μL), 2 (34 μL), 3 (34 μL), 4 (4 μL), 6 (4 μL), 10 (4 μL), 15 (2 μL), 16 (2 μL) | 39 μL | 200 μL |
| c4.0B.core | latch design 1, no aptamers | 1 (77 μL), 2 (34 μL), 3 (34 μL), 4 (4 μL), 6 (4 μL) | 47 μL | 200 μL |
| c4.0B.latches | latch design 1, no aptamers | 8 (4 μL), 11 (2 μL), 12 (2 μL) | - | - |
| c4.0C.core | latch design 2, no aptamers | 1 (77 μL), 2 (34 μL), 3 (34 μL), 4 (4 μL), 6 (4 μL) | 47 μL | 200 μL |
| c4.0C.latches | latch design 2, no aptamers | 9 (4 μL), 13 (2 μL), 14 (2 μL), 23 (4 μL), 24 (2 μL), 25 (2 μL) | - | - |
2. Protocol
- mixed 10 μL of each 50 μM stock oligo to make pre-working stocks - mixed listed pre-working stocks to make working stocks where final concentration of each oligo is 250 nM
