IGEM:Harvard/2006/vlau/Notebook/2006-7-27: Difference between revisions
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7 = c4.0Fb 2x PEG pellet | 7 = c4.0Fb 2x PEG pellet | ||
8 = c4.0Fb 2x PEG supernatant | 8 = c4.0Fb 2x PEG supernatant | ||
9 =empty | 9 = empty | ||
10 = c4.0Fb 1x PEG pellet | 10 = c4.0Fb 1x PEG pellet | ||
11 = c4.0Fb 1x PEG supernatant | 11 = c4.0Fb 1x PEG supernatant | ||
Revision as of 08:36, 27 July 2006
PEG precipitation (cont'd)
1. Protocol
- ran 3 samples + supernantants on 2.0% agarose gel w/ 11 mM MgCl2
- Lanes:
1 = ladder
2 = c4.0Fb folding reaction
3 = c4.0Fb 1x PEG pellet
4 = c4.0Fb 1x PEG supernatant
5 = c4.0Fb 1.5x PEG pellet
6 = c4.0Fb 1.5x PEG supernatant
7 = c4.0Fb 2x PEG pellet
8 = c4.0Fb 2x PEG supernatant
9 = empty
10 = c4.0Fb 1x PEG pellet
11 = c4.0Fb 1x PEG supernatant
12 = c4.0Fb 1.5x PEG pellet
13 = c4.0Fb 1.5x PEG supernatant
14 = c4.0Fb 2x PEG pellet
15 = c4.0Fb 2x PEG supernatant
2. Image
File:Peg precipitation trial 2.jpg
c5.0 Folding
1. Working Stock
| Stock ID | Experiment | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | |
| c5.0A | no latches, no aptamers | x | x | x | x | x | x | x |
2. Pre-Working Stocks
| Description | Plate Locations | Total |
| c5.0.1: core barrel oligos | box_v5_A; box_v5_B rows A-F (1-12), row G (1-4) | 172 |
| c5.0.2: top lid oligos | box_v5_C; box_v5_D row A (1-2) | 98 |
| c5.0.3: bottom lid oligos | box_v5_D row A (3-12), rows B-H (1-12); box_v5_E row A (1-4) | 98 |
| c5.0.4: barrel oligos @ outside aptamers -aptamers | box_v5_E row A (5-9) | 5 |
| c5.0.5: barrel oligos @ inside aptamers -aptamers | box_v5_E row A (10-12), row B (1-2) | 5 |
| c5.0.6: barrel oligos @ top latches -latches | box_v5_E row B (3-5) | 3 |
| c5.0.7: barrel oligos @ bottom latches -latches | box_v5_E row B (6-8) | 3 |
| Working Stocks | Description | Pre-Working Stocks | Water | Total |
| c5.0A | no latches, no aptamers | 1 (86 μL), 2 (49 μL), 3 (49 μL), 4 (2.5 μL), 5 (2.5 μL), 6 (1.5 μL), 7 (1.5 μL) | 8 μL | 200 μL |
