IGEM:Harvard/2007/Brainstorming/: Difference between revisions
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Brainstorming of Possible Adhesion Targets (feel free to add/reorganize/whatever--I just thought I'd get this started): | Brainstorming of Possible Adhesion Targets (feel free to add/reorganize/whatever--I just thought I'd get this started): | ||
'''textiles''' | |||
*silk | |||
*wool | |||
*polyester | |||
*nylon fibres | |||
*Could use the MIT idea to clone E coli that secrete pleasant smells and similarly bind to these fibers -- a living deodorant! | |||
'''eukaryotic cells''' | |||
*Mosquito gut epithelial cells as targets | *Mosquito gut epithelial cells as targets | ||
'''other mammalian''' | '''other mammalian''' | ||
*Laminins | *Laminins | ||
*fibronectin | *fibronectin | ||
'''Mammalian cell surface proteins''' | '''Mammalian cell surface proteins''' | ||
*gp75 - Surface expression of gp75 on mouse melanoma cells correlates with the ability of a monoclonal antibody against gp75 to reject melanomas in syngeneic mice | *gp75 - Surface expression of gp75 on mouse melanoma cells correlates with the ability of a monoclonal antibody against gp75 to reject melanomas in syngeneic mice | ||
'''Protozoan membrane proteins''' | '''Protozoan membrane proteins''' | ||
| Line 35: | Line 46: | ||
*Epstein Barr LMP1/LMP2 | *Epstein Barr LMP1/LMP2 | ||
*HIV gp120 | *HIV gp120 | ||
'''Cancer Membrane Proteins''' | '''Cancer Membrane Proteins''' | ||
| Line 41: | Line 53: | ||
*NGEP-L | *NGEP-L | ||
*[http://clincancerres.aacrjournals.org/cgi/content/abstract/7/12/4182?ck=nck HP59] | *[http://clincancerres.aacrjournals.org/cgi/content/abstract/7/12/4182?ck=nck HP59] | ||
'''Assorted''' | '''Assorted''' | ||
| Line 49: | Line 62: | ||
== 2 Component Systems == | == 2 Component Systems == | ||
* We | * We should start researching these as well as the tagets. | ||
* Perhaps The TF's (myself included, of course) can help point out some papers with which to start. | * Perhaps The TF's (myself included, of course) can help point out some papers with which to start. | ||
- Nick | - Nick | ||
Revision as of 06:59, 15 May 2007
Adhesion Targets
Brainstorming of Possible Adhesion Targets (feel free to add/reorganize/whatever--I just thought I'd get this started):
textiles
- silk
- wool
- polyester
- nylon fibres
- Could use the MIT idea to clone E coli that secrete pleasant smells and similarly bind to these fibers -- a living deodorant!
eukaryotic cells
- Mosquito gut epithelial cells as targets
other mammalian
- Laminins
- fibronectin
Mammalian cell surface proteins
- gp75 - Surface expression of gp75 on mouse melanoma cells correlates with the ability of a monoclonal antibody against gp75 to reject melanomas in syngeneic mice
Protozoan membrane proteins
- By the way, I was thinking about expressing plasmodium falciparum proteins on the surface of E coli and maybe showing somehow that an immune rxn can be elicited in mice or sthing like that e.g. in humans, "High levels of plasma chitotriosidase are a marker of macrophage activation..."
- Speaking on targets, heregoes:
- MSP-1 --> well characterized
- CSP
- EBA-175, a 175 kDa 'erythrocyte binding antigen' from P. falciparum
- DBP, Duffy-binding protein from P. vivax and P. knowlesi
- SSP2, Plasmodium sporozoite surface protein-2. Also known as TRAP (thrombospondin-related adhesive protein).
- Proteins with homology to SSP2/TRAP from Toxoplasma (MIC2), Eimeria (Etp100), and Cryptosporidium
- CTRP, circumsporozoite- and TRAP-related protein of Plasmodium found in the ookinete stage
- (see http://www.tulane.edu/~wiser/malaria/cmb.html#junction)
- MSP-1 polypeptide fragments
Viral Membrane Proteins
- Influenza A M2 Protein
- Neuraminidase
- Hemaglutinin
- Semliki Forest membrane proteins
- Epstein Barr LMP1/LMP2
- HIV gp120
Cancer Membrane Proteins
- Eag1 potassium channels
- Muc1
- NGEP-L
- HP59
Assorted
- Presenilin
2 Component Systems
- We should start researching these as well as the tagets.
- Perhaps The TF's (myself included, of course) can help point out some papers with which to start.
- Nick
Cascade Network Structure
- Some loose brainstorming on a possible reporter network that can be activated from the membrane protein as we discussed would be beneficial as well, if we plan to do something like that.
- May be good to start with something similar to what has been done previously and hook it up to GFP.
- Some TF direction would be warranted here as well.
- Nick
Previous Brainstorming Ideas
Btw, can we reorganize the brainstorming page to put the older brainstorming into folders or something and only have the stuff relevant to our project still on the front page? I have no idea how to do this...thanks. -Shaunak
Hope you like the formatting.
--Stephanie (5/12)
Brainstorming, 4/23 to 5/9
Misc Brainstorming, pre-5/9
Brainstorming from Second Meeting, 4/05
Brainstorming from First Meeting, 3/19
