IGEM:Harvard/2007/Gels: Difference between revisions
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[[Image: Week 0 Plasmid Gel.jpg|thumb| | [[Image: Week 0 Plasmid Gel.jpg|thumb|250px]] | ||
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[[Image:June 19 Gel.jpg|thumb| | [[Image:June 19 Gel.jpg|thumb|250px]]<br> | ||
Description: 6/20 gel<br> | |||
Well 1 Ladder (10 kb) | Well 1 Ladder (10 kb) | ||
<br> 27.5 uL each of: <br> | <br> 27.5 uL each of: <br> | ||
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[[Image:6-19-07_gel_after_excision.jpg|thumb| | [[Image:6-19-07_gel_after_excision.jpg|thumb|250px]] | ||
Description: 6/20 gel after excision<br> | |||
Revision as of 08:50, 20 June 2007
Well 1 Ladder
Well 2 OmpA1 in PET
Well 3 OmpA2 in PET
Well 4 OmpA1 +his tag in PET
Well 5 OmpA1 +strep tag in PET
Well 6 OmpA2 +his tag in PET
Well 7 OmpA2 +strep tag in PET
Description: 6/20 gel
Well 1 Ladder (10 kb)
27.5 uL each of:
Well 2 OmpA1, Nhe1, Pst1, Group 1
Well 3 OmpA1, Nhe1, Pst1, Group 1
Well 4 OmpA1, Nhe1, Pst1, Group 2
Well 5 OmpA1, Nhe1, Pst1, Group 2
Well 6 OmpA1, Nhe1, Pst1, Group 2
Well 7 OmpA2, Nhe1, Pst1, Group 4
Well 8 OmpA2, Nhe1, Pst1, Group 4
Well 9 OmpA2, Nhe1, Pst1, Group 3
Well 10 OmpA2, Nhe1, Pst1, Perry's
Well 11 OmpA2, Nhe1, Pst1, Perry's
See Notebook page for gel extraction weights and values.
Description: 6/20 gel after excision
