IGEM:Harvard/2007/Gels: Difference between revisions
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Well 6 OmpA2 +his tag in PET <br> | Well 6 OmpA2 +his tag in PET <br> | ||
Well 7 OmpA2 +strep tag in PET <br> | Well 7 OmpA2 +strep tag in PET <br> | ||
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[[Image:6-22-07_overexpression_gel.jpg]] |
Revision as of 08:50, 22 June 2007
Description: Week 0 gel
Well 1 Ladder
Well 2 OmpA1 in PET
Well 3 OmpA2 in PET
Well 4 OmpA1 +his tag in PET
Well 5 OmpA1 +strep tag in PET
Well 6 OmpA2 +his tag in PET
Well 7 OmpA2 +strep tag in PET
Description: 6/20 gel
Well 1 Ladder (10 kb)
27.5 uL each of:
Well 2 OmpA1, Nhe1, Pst1, Group 1
Well 3 OmpA1, Nhe1, Pst1, Group 1
Well 4 OmpA1, Nhe1, Pst1, Group 2
Well 5 OmpA1, Nhe1, Pst1, Group 2
Well 6 OmpA1, Nhe1, Pst1, Group 2
Well 7 OmpA2, Nhe1, Pst1, Group 4
Well 8 OmpA2, Nhe1, Pst1, Group 4
Well 9 OmpA2, Nhe1, Pst1, Group 3
Well 10 OmpA2, Nhe1, Pst1, Perry's
Well 11 OmpA2, Nhe1, Pst1, Perry's
See Notebook page for gel extraction weights and values.
Description: 6/22 gel
Description:
Well 1 Ladder
Well 2 OmpA1 in PET
Well 3 OmpA2 in PET
Well 4 OmpA1 +his tag in PET
Well 5 OmpA1 +strep tag in PET
Well 6 OmpA2 +his tag in PET
Well 7 OmpA2 +strep tag in PET