IGEM:Harvard/2007/Gels

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Line 1: Line 1:
[[Image: Week 0 Plasmid Gel.jpg|thumb|left|250px]]
[[Image: Week 0 Plasmid Gel.jpg|thumb|left|250px]]
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<center>
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====Description: Week 0 gel====
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Description: Week 0 gel
Well 1 Ladder <br>
Well 1 Ladder <br>
Well 2 OmpA1 in PET <br>
Well 2 OmpA1 in PET <br>
Line 10: Line 10:
Well 6 OmpA2 +his tag in PET <br>
Well 6 OmpA2 +his tag in PET <br>
Well 7 OmpA2 +strep tag in PET <br>
Well 7 OmpA2 +strep tag in PET <br>
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</center>
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 +
 
 +
 
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[[Image:June 19 Gel.jpg|thumb|left|250px]]<br>
[[Image:June 19 Gel.jpg|thumb|left|250px]]<br>
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====Description: 6/20 gel====
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Description: 6/20 gel<br>
Well 1 Ladder (10 kb)
Well 1 Ladder (10 kb)
<br> 27.5 uL each of: <br>
<br> 27.5 uL each of: <br>
Line 37: Line 39:
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====Description: 6/19 gel after excision====
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Description: 6/19 gel after excision <br>
 +
 
Well 1 Ladder <br>
Well 1 Ladder <br>
Well 2 OmpA1 in PET <br>
Well 2 OmpA1 in PET <br>
Line 53: Line 56:
[[Image:6-22-07_overexpression_gel.jpg|thumb|left|250px]]
[[Image:6-22-07_overexpression_gel.jpg|thumb|left|250px]]
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====Description: 6/22 overexpression gel====
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Description: 6/22 overexpression gel <br>
   
   
lane 1 Ladder <br>
lane 1 Ladder <br>
Line 71: Line 74:
[[Image:6-22-07_overexpressgel2_laddercontaminate.jpg|thumb|left|250px]]
[[Image:6-22-07_overexpressgel2_laddercontaminate.jpg|thumb|left|250px]]
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====Description: Contaminated 6/22 overexpression gel====
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Description: Contaminated 6/22 overexpression gel <br>
Explanation: Failed gel because of contamination in each lane by the ladder, which was mistakenly added.
Explanation: Failed gel because of contamination in each lane by the ladder, which was mistakenly added.

Revision as of 12:17, 22 June 2007


Description: Week 0 gel Well 1 Ladder
Well 2 OmpA1 in PET
Well 3 OmpA2 in PET
Well 4 OmpA1 +his tag in PET
Well 5 OmpA1 +strep tag in PET
Well 6 OmpA2 +his tag in PET
Well 7 OmpA2 +strep tag in PET





Description: 6/20 gel
Well 1 Ladder (10 kb)
27.5 uL each of:
Well 2 OmpA1, Nhe1, Pst1, Group 1
Well 3 OmpA1, Nhe1, Pst1, Group 1
Well 4 OmpA1, Nhe1, Pst1, Group 2
Well 5 OmpA1, Nhe1, Pst1, Group 2
Well 6 OmpA1, Nhe1, Pst1, Group 2
Well 7 OmpA2, Nhe1, Pst1, Group 4
Well 8 OmpA2, Nhe1, Pst1, Group 4
Well 9 OmpA2, Nhe1, Pst1, Group 3
Well 10 OmpA2, Nhe1, Pst1, Perry's
Well 11 OmpA2, Nhe1, Pst1, Perry's
See Notebook page for gel extraction weights and values.



Description: 6/19 gel after excision

Well 1 Ladder
Well 2 OmpA1 in PET
Well 3 OmpA2 in PET
Well 4 OmpA1 +his tag in PET
Well 5 OmpA1 +strep tag in PET
Well 6 OmpA2 +his tag in PET
Well 7 OmpA2 +strep tag in PET




Description: 6/22 overexpression gel

lane 1 Ladder
lane 2 OmpA2 & strep non-induced
lane 3 OmpA2 & his induced immediately
lane 4 OmpA1 & strep induced immediately
lane 5 OmpA1 & his induced immediately PET
lane 6 OmpA2 & strep non-induced
lane 7 OmpA2 & his non-induced





Description: Contaminated 6/22 overexpression gel

Explanation: Failed gel because of contamination in each lane by the ladder, which was mistakenly added.

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