IGEM:Harvard/2007/Gels

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[[Image: Week 0 Plasmid Gel.jpg|thumb|left|250px]]
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== Week 0 gel ==
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[[Image: Week 0 Plasmid Gel.jpg|thumb|left|250px]]<br>
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Description: Week 0 gel<br>
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Description: Week 0 gel
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Well 1 Ladder <br>
Well 1 Ladder <br>
Well 2 OmpA1 in PET <br>
Well 2 OmpA1 in PET <br>
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Well 5 OmpA1 +strep tag in PET  <br>
Well 5 OmpA1 +strep tag in PET  <br>
Well 6 OmpA2 +his tag in PET <br>
Well 6 OmpA2 +his tag in PET <br>
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Well 7 OmpA2 +strep tag in PET <br>
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Well 7 OmpA2 +strep tag in PET <br><br><br><br>
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== 6/20 gel ==
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[[Image:June 19 Gel.jpg|thumb|left|250px]]
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[[Image:June 19 Gel.jpg|thumb|left|250px]]<br>
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Description: 6/20 gel<br>
Description: 6/20 gel<br>
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Well 1 Ladder (10 kb)
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Well 1 Ladder (10 kb) <br>
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<br> 27.5 uL each of: <br>
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Well 2 OmpA1, Nhe1, Pst1, Group 1 27.5 uL<br>
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Well 2 OmpA1, Nhe1, Pst1, Group 1 <br>
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Well 3 OmpA1, Nhe1, Pst1, Group 1 27.5 uL<br>
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Well 3 OmpA1, Nhe1, Pst1, Group 1 <br>
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Well 4 OmpA1, Nhe1, Pst1, Group 2 27.5 uL<br>
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Well 4 OmpA1, Nhe1, Pst1, Group 2 <br>
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Well 5 OmpA1, Nhe1, Pst1, Group 2 27.5 uL<br>
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Well 5 OmpA1, Nhe1, Pst1, Group 2 <br>
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Well 6 OmpA1, Nhe1, Pst1, Group 2 27.5 uL<br>
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Well 6 OmpA1, Nhe1, Pst1, Group 2 <br>
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Well 7 OmpA2, Nhe1, Pst1, Group 4 27.5 uL<br>
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Well 7 OmpA2, Nhe1, Pst1, Group 4 <br>
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Well 8 OmpA2, Nhe1, Pst1, Group 4 27.5 uL<br>
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Well 8 OmpA2, Nhe1, Pst1, Group 4 <br>
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Well 9 OmpA2, Nhe1, Pst1, Group 3 27.5 uL<br>
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Well 9 OmpA2, Nhe1, Pst1, Group 3 <br>
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Well 10 OmpA2, Nhe1, Pst1, Perry's 27.5 uL<br>
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Well 10 OmpA2, Nhe1, Pst1, Perry's<br>
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Well 11 OmpA2, Nhe1, Pst1, Perry's<br>
Well 11 OmpA2, Nhe1, Pst1, Perry's<br>
See Notebook page for gel extraction weights and values.<br>
See Notebook page for gel extraction weights and values.<br>
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== 6/19 gel after excision==
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[[Image:6-19-07_gel_after_excision.jpg|thumb|left|250px]]<br>
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[[Image:6-19-07_gel_after_excision.jpg|thumb|left|250px]]
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Description: 6/19 gel after excision <br>
Description: 6/19 gel after excision <br>
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Well 5 OmpA1 +strep tag in PET  <br>
Well 5 OmpA1 +strep tag in PET  <br>
Well 6 OmpA2 +his tag in PET <br>
Well 6 OmpA2 +his tag in PET <br>
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Well 7 OmpA2 +strep tag in PET <br>
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Well 7 OmpA2 +strep tag in PET <br><br><br><br>
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== 6/22 overexpression gel ==
[[Image:6-22-07_overexpression_gel.jpg|thumb|left|250px]]
[[Image:6-22-07_overexpression_gel.jpg|thumb|left|250px]]
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Description: 6/22 overexpression gel <br>
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<br>Description: 6/22 overexpression gel <br>
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lane 1 Ladder <br>
lane 1 Ladder <br>
lane 2 OmpA1 + his6 non-induced<br>
lane 2 OmpA1 + his6 non-induced<br>
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lane 7 OmpA2 + his6 induced <br>
lane 7 OmpA2 + his6 induced <br>
lane 8 OmpA2 + strep2 non-induced <br>
lane 8 OmpA2 + strep2 non-induced <br>
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lane 9 OmpA2 + strep2 induced <br>
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lane 9 OmpA2 + strep2 induced <br><br>
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== 6/22 overexpression gel2, ladder contaminated ==
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[[Image:6-22-07_overexpressgel2_laddercontaminate.jpg|thumb|left|250px]]
[[Image:6-22-07_overexpressgel2_laddercontaminate.jpg|thumb|left|250px]]
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<br><br><br>
Description: Contaminated 6/22 overexpression gel <br>
Description: Contaminated 6/22 overexpression gel <br>
Explanation: Failed gel because of contamination in each lane by the ladder, which was mistakenly added.
Explanation: Failed gel because of contamination in each lane by the ladder, which was mistakenly added.

Revision as of 12:33, 27 June 2007

Contents

Week 0 gel


Description: Week 0 gel
Well 1 Ladder
Well 2 OmpA1 in PET
Well 3 OmpA2 in PET
Well 4 OmpA1 +his tag in PET
Well 5 OmpA1 +strep tag in PET
Well 6 OmpA2 +his tag in PET
Well 7 OmpA2 +strep tag in PET



6/20 gel

Description: 6/20 gel
Well 1 Ladder (10 kb)
Well 2 OmpA1, Nhe1, Pst1, Group 1 27.5 uL
Well 3 OmpA1, Nhe1, Pst1, Group 1 27.5 uL
Well 4 OmpA1, Nhe1, Pst1, Group 2 27.5 uL
Well 5 OmpA1, Nhe1, Pst1, Group 2 27.5 uL
Well 6 OmpA1, Nhe1, Pst1, Group 2 27.5 uL
Well 7 OmpA2, Nhe1, Pst1, Group 4 27.5 uL
Well 8 OmpA2, Nhe1, Pst1, Group 4 27.5 uL
Well 9 OmpA2, Nhe1, Pst1, Group 3 27.5 uL
Well 10 OmpA2, Nhe1, Pst1, Perry's 27.5 uL
Well 11 OmpA2, Nhe1, Pst1, Perry's
See Notebook page for gel extraction weights and values.

6/19 gel after excision


Description: 6/19 gel after excision

Well 1 Ladder
Well 2 OmpA1 in PET
Well 3 OmpA2 in PET
Well 4 OmpA1 +his tag in PET
Well 5 OmpA1 +strep tag in PET
Well 6 OmpA2 +his tag in PET
Well 7 OmpA2 +strep tag in PET



6/22 overexpression gel


Description: 6/22 overexpression gel
lane 1 Ladder
lane 2 OmpA1 + his6 non-induced
lane 3 OmpA1 + his6 induced
lane 4 OmpA1 + strep2 non-induced
lane 5 OmpA1 + strep2 induced
lane 6 OmpA2 + his6 non-induced
lane 7 OmpA2 + his6 induced
lane 8 OmpA2 + strep2 non-induced
lane 9 OmpA2 + strep2 induced

6/22 overexpression gel2, ladder contaminated




Description: Contaminated 6/22 overexpression gel

Explanation: Failed gel because of contamination in each lane by the ladder, which was mistakenly added.

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