IGEM:Harvard/2007/Gels

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(6/22 overexpression gel2, ladder contaminated)
(6/27 plasmid gel, performed by Stephanie)
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Explanation:  
Explanation:  
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Wells are as follows
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For further information concerning amounts of plasmid in sample, please refer to Stephanie's notebook page (under Stephanie's personal page, shlo/notebook)
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Wells are as follows:<br>
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#Well 1: 10uL ladder of 1kB plus, from Invitrogen<br>
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<i> Note: this ladder cannot be used to quantify - perhaps a reason we should just use 1kB ladder, rather than 1kB+, in the future</i><br>
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#Well 2: 1uL OmpA1plasmid (diluted to 20uL)
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#Well 3: 3uL OmpA1plasmid (diluted to 20)
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#Well 4: 1uL OmpA2plasmid (diluted to 20)
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#Well 5: 3uL OmpA2plasmid (diluted to 20)

Revision as of 14:00, 27 June 2007

Contents

Week 0 gel


Description: Week 0 gel
Well 1 Ladder
Well 2 OmpA1 in PET
Well 3 OmpA2 in PET
Well 4 OmpA1 +his tag in PET
Well 5 OmpA1 +strep tag in PET
Well 6 OmpA2 +his tag in PET
Well 7 OmpA2 +strep tag in PET



6/20 gel

Description: 6/20 gel
Well 1 Ladder (10 kb)
Well 2 OmpA1, Nhe1, Pst1, Group 1 27.5 uL
Well 3 OmpA1, Nhe1, Pst1, Group 1 27.5 uL
Well 4 OmpA1, Nhe1, Pst1, Group 2 27.5 uL
Well 5 OmpA1, Nhe1, Pst1, Group 2 27.5 uL
Well 6 OmpA1, Nhe1, Pst1, Group 2 27.5 uL
Well 7 OmpA2, Nhe1, Pst1, Group 4 27.5 uL
Well 8 OmpA2, Nhe1, Pst1, Group 4 27.5 uL
Well 9 OmpA2, Nhe1, Pst1, Group 3 27.5 uL
Well 10 OmpA2, Nhe1, Pst1, Perry's 27.5 uL
Well 11 OmpA2, Nhe1, Pst1, Perry's
See Notebook page for gel extraction weights and values.

6/19 gel after excision


Description: 6/19 gel after excision

Well 1 Ladder
Well 2 OmpA1 in PET
Well 3 OmpA2 in PET
Well 4 OmpA1 +his tag in PET
Well 5 OmpA1 +strep tag in PET
Well 6 OmpA2 +his tag in PET
Well 7 OmpA2 +strep tag in PET



6/22 overexpression gel


Description: 6/22 overexpression gel
lane 1 Ladder
lane 2 OmpA1 + his6 non-induced
lane 3 OmpA1 + his6 induced
lane 4 OmpA1 + strep2 non-induced
lane 5 OmpA1 + strep2 induced
lane 6 OmpA2 + his6 non-induced
lane 7 OmpA2 + his6 induced
lane 8 OmpA2 + strep2 non-induced
lane 9 OmpA2 + strep2 induced

6/22 overexpression gel2, ladder contaminated




Description: Contaminated 6/22 overexpression gel

Explanation: Failed gel because of contamination in each lane by the ladder, which was mistakenly added.





6/27 plasmid gel, performed by Stephanie




Description: Gel using Lpp+OmpA1+pet29 and LppOmpA2+pet29

Explanation: For further information concerning amounts of plasmid in sample, please refer to Stephanie's notebook page (under Stephanie's personal page, shlo/notebook) Wells are as follows:

  1. Well 1: 10uL ladder of 1kB plus, from Invitrogen

Note: this ladder cannot be used to quantify - perhaps a reason we should just use 1kB ladder, rather than 1kB+, in the future

  1. Well 2: 1uL OmpA1plasmid (diluted to 20uL)
  2. Well 3: 3uL OmpA1plasmid (diluted to 20)
  3. Well 4: 1uL OmpA2plasmid (diluted to 20)
  4. Well 5: 3uL OmpA2plasmid (diluted to 20)
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