IGEM:Harvard/2007/Gels: Difference between revisions
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Explanation: Failed gel because of contamination in each lane by the ladder, which was mistakenly added. | Explanation: Failed gel because of contamination in each lane by the ladder, which was mistakenly added. | ||
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== 6/27 plasmid gel, performed by Stephanie == | == 6/27 plasmid gel, performed by Stephanie == |
Revision as of 11:57, 27 June 2007
Week 0 gel
Description: Week 0 gel
Well 1 Ladder
Well 2 OmpA1 in PET
Well 3 OmpA2 in PET
Well 4 OmpA1 +his tag in PET
Well 5 OmpA1 +strep tag in PET
Well 6 OmpA2 +his tag in PET
Well 7 OmpA2 +strep tag in PET
6/20 gel
Description: 6/20 gel
Well 1 Ladder (10 kb)
Well 2 OmpA1, Nhe1, Pst1, Group 1 27.5 uL
Well 3 OmpA1, Nhe1, Pst1, Group 1 27.5 uL
Well 4 OmpA1, Nhe1, Pst1, Group 2 27.5 uL
Well 5 OmpA1, Nhe1, Pst1, Group 2 27.5 uL
Well 6 OmpA1, Nhe1, Pst1, Group 2 27.5 uL
Well 7 OmpA2, Nhe1, Pst1, Group 4 27.5 uL
Well 8 OmpA2, Nhe1, Pst1, Group 4 27.5 uL
Well 9 OmpA2, Nhe1, Pst1, Group 3 27.5 uL
Well 10 OmpA2, Nhe1, Pst1, Perry's 27.5 uL
Well 11 OmpA2, Nhe1, Pst1, Perry's
See Notebook page for gel extraction weights and values.
6/19 gel after excision
Description: 6/19 gel after excision
Well 1 Ladder
Well 2 OmpA1 in PET
Well 3 OmpA2 in PET
Well 4 OmpA1 +his tag in PET
Well 5 OmpA1 +strep tag in PET
Well 6 OmpA2 +his tag in PET
Well 7 OmpA2 +strep tag in PET
6/22 overexpression gel
Description: 6/22 overexpression gel
lane 1 Ladder
lane 2 OmpA1 + his6 non-induced
lane 3 OmpA1 + his6 induced
lane 4 OmpA1 + strep2 non-induced
lane 5 OmpA1 + strep2 induced
lane 6 OmpA2 + his6 non-induced
lane 7 OmpA2 + his6 induced
lane 8 OmpA2 + strep2 non-induced
lane 9 OmpA2 + strep2 induced
6/22 overexpression gel2, ladder contaminated
Description: Contaminated 6/22 overexpression gel
Explanation: Failed gel because of contamination in each lane by the ladder, which was mistakenly added.
6/27 plasmid gel, performed by Stephanie
Description: Gel using Lpp+OmpA1+pet29 and LppOmpA2+pet29
Explanation: Wells are as follows