IGEM:Harvard/2007/Gels

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Contents

Description: Week 0 gel

Well 1 Ladder
Well 2 OmpA1 in PET
Well 3 OmpA2 in PET
Well 4 OmpA1 +his tag in PET
Well 5 OmpA1 +strep tag in PET
Well 6 OmpA2 +his tag in PET
Well 7 OmpA2 +strep tag in PET




Description: 6/20 gel

Well 1 Ladder (10 kb)
27.5 uL each of:
Well 2 OmpA1, Nhe1, Pst1, Group 1
Well 3 OmpA1, Nhe1, Pst1, Group 1
Well 4 OmpA1, Nhe1, Pst1, Group 2
Well 5 OmpA1, Nhe1, Pst1, Group 2
Well 6 OmpA1, Nhe1, Pst1, Group 2
Well 7 OmpA2, Nhe1, Pst1, Group 4
Well 8 OmpA2, Nhe1, Pst1, Group 4
Well 9 OmpA2, Nhe1, Pst1, Group 3
Well 10 OmpA2, Nhe1, Pst1, Perry's
Well 11 OmpA2, Nhe1, Pst1, Perry's
See Notebook page for gel extraction weights and values.



Description: 6/19 gel after excision

Well 1 Ladder
Well 2 OmpA1 in PET
Well 3 OmpA2 in PET
Well 4 OmpA1 +his tag in PET
Well 5 OmpA1 +strep tag in PET
Well 6 OmpA2 +his tag in PET
Well 7 OmpA2 +strep tag in PET




Description: 6/22 overexpression gel

lane 1 Ladder
lane 2 OmpA2 & strep non-induced
lane 3 OmpA2 & his induced immediately
lane 4 OmpA1 & strep induced immediately
lane 5 OmpA1 & his induced immediately PET
lane 6 OmpA2 & strep non-induced
lane 7 OmpA2 & his non-induced





Description: Contaminated 6/22 overexpression gel

Explanation: Failed gel because of contamination in each lane by the ladder, which was mistakenly added.

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