IGEM:Harvard/2007/Laboratory Notebooks/Bacterial Targeting Protocol/Week 7: Difference between revisions
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(New page: ===Gel Purification of AIDA Digest (07/30/07)=== #Add 150 ml 1x TBS #Add 1.8 g of agarose #Heat to a boil, let cool to a warm/hot temperature. #Add 1.5 ul EtBr #Pour into gel and wait unti...) |
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#Pour into gel and wait until solidified. | #Pour into gel and wait until solidified. | ||
#Run gel at 100-150 V | #Run gel at 100-150 V | ||
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===Gel Purification of AIDA Digestl, Part 2 (07/30/07)=== | |||
1.2% agarose gel, 150V | |||
Lanes:<br> | |||
1. 1kb+ Ladder, 10ul<br> | |||
2. 1kb+ Ladder, 20ul<br> | |||
3. blank<br> | |||
4. AIDA1-PCR with random library, cut with Nde1 and Not1, Kevin's reaction<br> | |||
5. AIDA1-PCR with random library, cut with Nde1 and Not1, Kevin's reaction<br> | |||
6. blank<br> | |||
7. AIDA1-PCR with random library, cut with Nde1 and Not1, Alex's reaction<br> | |||
8. AIDA1-PCR with random library, cut with Nde1 and Not1, Alex's reaction<br> | |||
9. blank<br> | |||
10. AIDA1 expression plasmid 3<br> | |||
11. AIDA1 expression plasmid 3<br> | |||
12. blank<br> | |||
13. AIDA1 expression plasmid 4<br> | |||
14. blank<br> | |||
15. AIDA1 expression plasmid 5<br> | |||
16. blank<br> | |||
17. AIDA1 expression plasmid 6<br> | |||
18. blank<br> | |||
19. AIDA1 expression plasmid 7<br> | |||
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Revision as of 12:06, 30 July 2007
Gel Purification of AIDA Digest (07/30/07)
- Add 150 ml 1x TBS
- Add 1.8 g of agarose
- Heat to a boil, let cool to a warm/hot temperature.
- Add 1.5 ul EtBr
- Pour into gel and wait until solidified.
- Run gel at 100-150 V
Gel Purification of AIDA Digestl, Part 2 (07/30/07)
1.2% agarose gel, 150V
Lanes:
1. 1kb+ Ladder, 10ul
2. 1kb+ Ladder, 20ul
3. blank
4. AIDA1-PCR with random library, cut with Nde1 and Not1, Kevin's reaction
5. AIDA1-PCR with random library, cut with Nde1 and Not1, Kevin's reaction
6. blank
7. AIDA1-PCR with random library, cut with Nde1 and Not1, Alex's reaction
8. AIDA1-PCR with random library, cut with Nde1 and Not1, Alex's reaction
9. blank
10. AIDA1 expression plasmid 3
11. AIDA1 expression plasmid 3
12. blank
13. AIDA1 expression plasmid 4
14. blank
15. AIDA1 expression plasmid 5
16. blank
17. AIDA1 expression plasmid 6
18. blank
19. AIDA1 expression plasmid 7