IGEM:Harvard/2007/Laboratory Notebooks/Bacterial Targeting Protocol/Week 8

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(New page: ===MACS Assay 1- his and strep AIDA sender cells (08/05/07)=== Purpose: Testing the AIDA sender cell constructs. #OD equilibriated to 0.3 for induced #Followed [[IGEM:Harvard/2007/Protoc...)
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Revision as of 13:20, 10 August 2007

MACS Assay 1- his and strep AIDA sender cells (08/05/07)

Purpose: Testing the AIDA sender cell constructs.

  1. OD equilibriated to 0.3 for induced
  2. Followed Indirect Magnetic Labeling Protocol
    1. Used 50 ul of Strep/His and 50 ul of RFP Bacteria
    2. Used 8 ul his antibodies and 16 ul strep antibodies
    3. Used 18 ul of Beads
  3. Then follow Magnetic Separation
    1. Use the additional measures

MACS Assay 2- his and strep AIDA sender cells (08/05/07)

Purpose: Testing the AIDA sender cell constructs.

  1. OD equilibriated to 0.5 for induced
  2. Followed Indirect Magnetic Labeling Protocol
    1. Used 25 ul of Strep/His and 25 ul of RFP Bacteria
    2. Used 8 ul his antibodies and 16 ul strep antibodies
    3. Used 18 ul of Beads
  3. Then follow Magnetic Separation
    1. Use the additional measures

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