IGEM:Harvard/2007/Laboratory Notebooks/Bacterial Targeting Protocol/Week 8

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(New page: ===MACS Assay 1- his and strep AIDA sender cells (08/05/07)=== Purpose: Testing the AIDA sender cell constructs. #OD equilibriated to 0.3 for induced #Followed [[IGEM:Harvard/2007/Protoc...)
(MACS Assay 2- his and strep AIDA sender cells (08/05/07))
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===MACS Assay 2 results (08/11/07)===
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See [[IGEM:Harvard/2007/Plates|Colony Counts]] for exact numbers of colonies.
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Observation:  Assay was successful, and we were able to select for the red colonies over the white background colonies.
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Conclusion:  We have a strong construct that seems to be working that we can try to incorporate with the Quorum Sensing group.
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Revision as of 15:23, 11 August 2007

MACS Assay 1- his and strep AIDA sender cells (08/05/07)

Purpose: Testing the AIDA sender cell constructs.

  1. OD equilibriated to 0.3 for induced
  2. Followed Indirect Magnetic Labeling Protocol
    1. Used 50 ul of Strep/His and 50 ul of RFP Bacteria
    2. Used 8 ul his antibodies and 16 ul strep antibodies
    3. Used 18 ul of Beads
  3. Then follow Magnetic Separation
    1. Use the additional measures

MACS Assay 2- his and strep AIDA sender cells (08/05/07)

Purpose: Testing the AIDA sender cell constructs.

  1. OD equilibriated to 0.5 for induced
  2. Followed Indirect Magnetic Labeling Protocol
    1. Used 25 ul of Strep/His and 25 ul of RFP Bacteria
    2. Used 8 ul his antibodies and 16 ul strep antibodies
    3. Used 18 ul of Beads
  3. Then follow Magnetic Separation
    1. Use the additional measures

MACS Assay 2 results (08/11/07)

See Colony Counts for exact numbers of colonies.

Observation: Assay was successful, and we were able to select for the red colonies over the white background colonies.

Conclusion: We have a strong construct that seems to be working that we can try to incorporate with the Quorum Sensing group.


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