IGEM:Harvard/2007/Laboratory Notebooks/Bacterial Targeting Protocol/Week 8: Difference between revisions
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(New page: ===MACS Assay 1- his and strep AIDA sender cells (08/05/07)=== Purpose: Testing the AIDA sender cell constructs. #OD equilibriated to 0.3 for induced #Followed [[IGEM:Harvard/2007/Protoc...) |
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===MACS Assay 2 results (08/11/07)=== | |||
See [[IGEM:Harvard/2007/Plates|Colony Counts]] for exact numbers of colonies. | |||
Observation: Assay was successful, and we were able to select for the red colonies over the white background colonies. | |||
Conclusion: We have a strong construct that seems to be working that we can try to incorporate with the Quorum Sensing group. | |||
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Revision as of 12:23, 11 August 2007
MACS Assay 1- his and strep AIDA sender cells (08/05/07)
Purpose: Testing the AIDA sender cell constructs.
- OD equilibriated to 0.3 for induced
- Followed Indirect Magnetic Labeling Protocol
- Used 50 ul of Strep/His and 50 ul of RFP Bacteria
- Used 8 ul his antibodies and 16 ul strep antibodies
- Used 18 ul of Beads
- Then follow Magnetic Separation
- Use the additional measures
MACS Assay 2- his and strep AIDA sender cells (08/05/07)
Purpose: Testing the AIDA sender cell constructs.
- OD equilibriated to 0.5 for induced
- Followed Indirect Magnetic Labeling Protocol
- Used 25 ul of Strep/His and 25 ul of RFP Bacteria
- Used 8 ul his antibodies and 16 ul strep antibodies
- Used 18 ul of Beads
- Then follow Magnetic Separation
- Use the additional measures
MACS Assay 2 results (08/11/07)
See Colony Counts for exact numbers of colonies.
Observation: Assay was successful, and we were able to select for the red colonies over the white background colonies.
Conclusion: We have a strong construct that seems to be working that we can try to incorporate with the Quorum Sensing group.
