IGEM:Harvard/2007/Laboratory Notebooks/Bacterial Targeting Protocol/Week 9

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Transformation of OMPA loop his and strep (8/14/07)

  1. Add 1 µl of the DNA solution directly to the cells. Stir gently to mix.
  2. Place the tubes on ice for a little more than 5 minutes
  3. Heat the tubes for about 45 seconds in a 42°C water bath; do not shake
  4. Place on ice for 2 minutes
  5. Add 250 µl of room temperature SOC Medium to each tube
  6. Shake at 37°C (300 rpm) for more than 30 minutes.
  7. Use sterile spreaders to spread the bacteria on an LB Carb plate.

Liquid Cultures for OMPA loop strep (8/17/07)

  1. Prepared liquid cultures
    1. 5 ml LB
    2. 5 ul amp
    3. single colony

Streak Plate for OMPA loop his (8/17/07)

  1. Used multiple streaks to dilute the plate, which was a lawn of bacteria with nearly indistinguishable colonies.
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