IGEM:Harvard/2007/Laboratory Notebooks/Quorum Sensing/Week 2: Difference between revisions
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#Put 150 mL of LB and 150 µL of ampicillin (all the parts are ampicillin resistant) into each flask | #Put 150 mL of LB and 150 µL of ampicillin (all the parts are ampicillin resistant) into each flask | ||
#Put into the agitating incubator overnight | #Put into the agitating incubator overnight | ||
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=== 6/27/07 === | === 6/27/07 === | ||
Revision as of 12:25, 27 June 2007
6/25/07
Transformation of Parts into Top10
See Perry's Transformation Protocol, transforming the parts F1610, I13263, and I13272 into Top10 cells
Plating of Top10 cells containing the parts
We plated the three parts onto three separate petri dishes.
6/26/07
Growth of colonies in liquid cultures
We grew a large amount because we want to carry out a midiprep tomorrow
- Take 3 500 mL flasks from the cabinet in the big room with the house on the chalkboard and the person saying "Yaye"
- Put 150 mL of LB and 150 µL of ampicillin (all the parts are ampicillin resistant) into each flask
- Put into the agitating incubator overnight
6/27/07
Midiprep with the liquid cultures grown on 6/26
See HiSpeed Midi and Maxi Prep for protocol used
