IGEM:Harvard/2007/Laboratory Notebooks/Quorum Sensing/Week 2: Difference between revisions

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==== Midiprep with the liquid cultures grown on 6/26====
==== Midiprep with the liquid cultures grown on 6/26====
See [[IGEM:Harvard/2007/Protocols/HiSpeed Midi and Maxi Prep|HiSpeed Midi and Maxi Prep]] for protocol used
See [[IGEM:Harvard/2007/Protocols/HiSpeed Midi and Maxi Prep|HiSpeed Midi and Maxi Prep]] for protocol used
==== Transformation of I13263 into BL21 ====
See [[IGEM:Harvard/2007/week_1#Transformation_protocol|Transformation Protocol]] for the protocol used.
==== Sequencing the I13263 ====
See [[IGEM:Harvard/2007/week_2#Sequencing_DNA|Sequencing Protocol]] for the protocol used.
Details of the order:
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Tube Label'''
| align="center" style="background:#f0f0f0;"|'''DNA Name'''
| align="center" style="background:#f0f0f0;"|'''DNA Length'''
| align="center" style="background:#f0f0f0;"|'''Primer'''
|-
| AV01||F1610||3987||VF2
|-
| AV02||F1610||3987||VR
|-
| AV03||I13263||4123||VF2
|-
| AV04||I13263||4123||VR
|-
| AV05||I13272||4162||VF2
|-
| AV06||I13272||4162||VR
|-
|
|}

Revision as of 19:00, 27 June 2007

6/25/07

Transformation of Parts into Top10

See Perry's Transformation Protocol, transforming the parts F1610, I13263, and I13272 into Top10 cells

Plating of Top10 cells containing the parts

We plated the three parts onto three separate petri dishes.

6/26/07

Growth of colonies in liquid cultures

We grew a large amount because we want to carry out a midiprep tomorrow

  1. Take 3 500 mL flasks from the cabinet in the big room with the house on the chalkboard and the person saying "Yaye"
  2. Put 150 mL of LB and 150 µL of ampicillin (all the parts are ampicillin resistant) into each flask
  3. Put into the agitating incubator overnight

6/27/07

Midiprep with the liquid cultures grown on 6/26

See HiSpeed Midi and Maxi Prep for protocol used

Transformation of I13263 into BL21

See Transformation Protocol for the protocol used.

Sequencing the I13263

See Sequencing Protocol for the protocol used.

Details of the order:

Tube Label DNA Name DNA Length Primer
AV01 F1610 3987 VF2
AV02 F1610 3987 VR
AV03 I13263 4123 VF2
AV04 I13263 4123 VR
AV05 I13272 4162 VF2
AV06 I13272 4162 VR
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