IGEM:Harvard/2007/Laboratory Notebooks/Quorum Sensing/Week 4: Difference between revisions
| Line 70: | Line 70: | ||
*[http://parts.mit.edu/registry/index.php/Part:BBa_I13273 I13273] | *[http://parts.mit.edu/registry/index.php/Part:BBa_I13273 I13273] | ||
**CORRECT! | **CORRECT! | ||
*[http://parts.mit.edu/registry/index.php/Part:BBa_J23039] > [http://parts.mit.edu/registry/index.php/Part:BBa_T9002] | *[http://parts.mit.edu/registry/index.php/Part:BBa_J23039 J23039] > [http://parts.mit.edu/registry/index.php/Part:BBa_T9002 T9002] | ||
**CORRECT | **CORRECT | ||
*[http://parts.mit.edu/registry/index.php/Part:BBa_S03608 S03608] > [http://parts.mit.edu/registry/index.php/Part:BBa_I13507 I13507] | *[http://parts.mit.edu/registry/index.php/Part:BBa_S03608 S03608] > [http://parts.mit.edu/registry/index.php/Part:BBa_I13507 I13507] | ||
**CORRECT! | **CORRECT! | ||
*[http://parts.mit.edu/registry/index.php/Part:BBa_S03623 S03623] > [http://parts.mit.edu/registry/index.php/Part:BBa_E0240] | *[http://parts.mit.edu/registry/index.php/Part:BBa_S03623 S03623] > [http://parts.mit.edu/registry/index.php/Part:BBa_E0240 E0240] | ||
**CORRECT! | **CORRECT! | ||
*[http://parts.mit.edu/registry/index.php/Part:BBa_S03623 S03623] > [http://parts.mit.edu/registry/index.php/Part:BBa_I13507] | *[http://parts.mit.edu/registry/index.php/Part:BBa_S03623 S03623] > [http://parts.mit.edu/registry/index.php/Part:BBa_I13507] | ||
**CORRECT! | **CORRECT! | ||
Revision as of 20:19, 12 July 2007
7/09/07
Colony PCR's
George, Stephanie, and Perry ran colony PCR's on the constructs that Perry and Stephanie transformed on the previous Friday.
The following colonies turned out to be correct:
- J06702, colony #2
- I6042, colony #1
- I6042, colony #2
- I13273, colony #2
- F2622, colony #1
- S03623 > I13507
- S03623> E0240
- S03608> I13507
- F2621, colony #1
- F2621, colony #2
7/10/07
Plate Reader
Stephanie made 1:20 dilutions into 200 and grew samples of T9002 (both {incubated overnight with 100nM OHHL} and {incubated overnight without induction}),
Unfortunately, the results/data for this experiment were lost due to Windows automatically restarting itself and not saving any data.
More Colony PCR's
Stephanie, Perry, and George ran more colony PCR's. The parts were split up into three gels, with different extension times for each gel group:
- Group 1: 1m15s extension
- Group 2: 2m15s extension
- Group 3: 3m15s extension
Results: The following parts ended up being correct:
- F2620 - colony #4
- F2620 - colony #5
- B0015 - colony #4
- F2620 > E0240 - colony #2
- J23039 > T9002 - colony #2
07/11/07
Setting Up the Overnight Fluorescent Plate Reader
Stephanie set up the plate reader for an overnight plate reader experiment with:
- T9002, I13273, and F2620 > E0240 all either induced with 100 nM or not induced
- J23039 > T9002
- B0015
- I13522
- I5211
- S03608 > I13507 w/ T9002
- S03608 > I13507 w/ I13273
All the above samples were 200 µL cultures of 1:100 dilutions of overnight liquid cultures. In addition, Stephanie ran six samples of the J23039 > T9002 that were prepared by dipping a colony swab into 200 µL of LB medium.
