IGEM:Harvard/2007/Meetings/Week 9: Difference between revisions
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[[Media: shlo0813igem.ppt | Stephanie's Powerpoint]] | |||
==Lunchtime meeting, 8/17== | |||
Harvard 2007 iGEM Jamboree Presentation Planning Meeting 1, 8/17 | |||
<br><br> | |||
<b><u>Content</u> | |||
<br><br> | |||
Introduction</b> | |||
*How cells physically interact with environment? | |||
*Underutilized aspects of iGEM: random libraries, surface expression | |||
*Figure of overall project: "Harvard iGEM 2007"; overall fusion of project | |||
<Br> | |||
<b>Targeting </b> | |||
*Random library | |||
*Loop/terminal | |||
*Targets | |||
**Nickel | |||
**Calmodulin | |||
**EGF | |||
**GST | |||
**His | |||
**Strep | |||
**PDZ | |||
*Figures: Plates: before and after enrichment, somehow present numbers (pie chart? bar graph?), microscopy images with beads and fluorescent cells accumulating | |||
<Br> | |||
<b>Cell-cell signaling</b> | |||
*QS – sender, receiver = biobricks, back to initial idea: target + QS, with figures | |||
*Figures: drop experiment (halo) | |||
<br><br> | |||
<B>Intracellular signaling</b> | |||
*Advantage of Fec system = gene expression with low level of background – need specificity with targets, etc, regulation on cell level rather than on population level | |||
*Figures: 3d structure, where and why we chose to alter loop region | |||
<br><Br> | |||
<B>Conclusion</b> | |||
*“Big goals” – medical applications | |||
*Self-regulated production of certain proteins – “preprogrammed assembly” | |||
*Constructs / futures | |||
<u><b>SLIDE allotment</b></u> | |||
*allow 45 seconds or so per slide | |||
*total of 15 ish slides | |||
*Intro = 2 slides | |||
**Slide 1: Why do we want to do this? | |||
**Slide 2: This is how we're going to do it; architecture of project | |||
*Targeting = 4 | |||
*Cell-cell (QS) = 4 | |||
*Intracellular signaling = 3 | |||
*Conclusion = 2 | |||
<u><b>TIME allotment</b></u> | |||
*Intro: 1 min | |||
*Targeting: 3 min | |||
*Cell-cell signaling: 2.5 min | |||
*Intracellular signaling: 2.5 min | |||
*Conclusion: 1 min |
Latest revision as of 10:35, 17 August 2007
Lunchtime meeting, 8/17
Harvard 2007 iGEM Jamboree Presentation Planning Meeting 1, 8/17
Content
Introduction
- How cells physically interact with environment?
- Underutilized aspects of iGEM: random libraries, surface expression
- Figure of overall project: "Harvard iGEM 2007"; overall fusion of project
Targeting
- Random library
- Loop/terminal
- Targets
- Nickel
- Calmodulin
- EGF
- GST
- His
- Strep
- PDZ
- Figures: Plates: before and after enrichment, somehow present numbers (pie chart? bar graph?), microscopy images with beads and fluorescent cells accumulating
Cell-cell signaling
- QS – sender, receiver = biobricks, back to initial idea: target + QS, with figures
- Figures: drop experiment (halo)
Intracellular signaling
- Advantage of Fec system = gene expression with low level of background – need specificity with targets, etc, regulation on cell level rather than on population level
- Figures: 3d structure, where and why we chose to alter loop region
Conclusion
- “Big goals” – medical applications
- Self-regulated production of certain proteins – “preprogrammed assembly”
- Constructs / futures
SLIDE allotment
- allow 45 seconds or so per slide
- total of 15 ish slides
- Intro = 2 slides
- Slide 1: Why do we want to do this?
- Slide 2: This is how we're going to do it; architecture of project
- Targeting = 4
- Cell-cell (QS) = 4
- Intracellular signaling = 3
- Conclusion = 2
TIME allotment
- Intro: 1 min
- Targeting: 3 min
- Cell-cell signaling: 2.5 min
- Intracellular signaling: 2.5 min
- Conclusion: 1 min