IGEM:Harvard/2007/Meetings/Week 9

Stephanie's Powerpoint

Lunchtime meeting, 8/17

Harvard 2007 iGEM Jamboree Presentation Planning Meeting 1, 8/17

Content

Introduction

• How cells physically interact with environment?
• Underutilized aspects of iGEM: random libraries, surface expression
• Figure of overall project: "Harvard iGEM 2007"; overall fusion of project

Targeting

• Random library
• Loop/terminal
• Targets
  • Nickel
  • Calmodulin
  • EGF
  • GST
  • His
  • Strep
  • PDZ
• Figures: Plates: before and after enrichment, somehow present numbers (pie chart? bar graph?), microscopy images with beads and fluorescent cells accumulating

Cell-cell signaling

• QS – sender, receiver = biobricks, back to initial idea: target + QS, with figures
• Figures: drop experiment (halo)

Intracellular signaling

• Advantage of Fec system = gene expression with low level of background – need specificity with targets, etc, regulation on cell level rather than on population level
• Figures: 3d structure, where and why we chose to alter loop region

Conclusion

• “Big goals” – medical applications
• Self-regulated production of certain proteins – “preprogrammed assembly”
• Constructs / futures

SLIDE allotment

• allow 45 seconds or so per slide
• total of 15 ish slides
• Intro = 2 slides
  • Slide 1: Why do we want to do this?
  • Slide 2: This is how we're going to do it; architecture of project
• Targeting = 4
• Cell-cell (QS) = 4
• Intracellular signaling = 3
• Conclusion = 2

TIME allotment

• Intro: 1 min
• Targeting: 3 min
• Cell-cell signaling: 2.5 min
• Intracellular signaling: 2.5 min
• Conclusion: 1 min