IGEM:Harvard/2007/Meetings/Week 9

Stephanie's Powerpoint

Lunchtime meeting, 8/17

Harvard 2007 iGEM Jamboree Presentation Planning Meeting 1, 8/17

Content

Introduction

• How cells physically interact with environment?
• Underutilized aspects of iGEM: random libraries, surface expression

Targeting

• Random library
• Loop/terminal
• Targets
  • Nickel
  • Calmodulin
  • EGF
  • GST
  • His
  • Strep
  • PDZ
• Figures: Plates: before and after enrichment, somehow present numbers (pie chart? bar graph?)

Cell-cell signaling

• QS – sender, receiver = biobricks, back to initial idea: target + QS, with figures
• Figures: drop experiment (halo)

Intracellular signaling

• Advantage of Fec system = gene expression with low level of background – need specificity with targets, etc, regulation on cell level rather than on population level
• Figures: 3d structure, where and why we chose to alter loop region

Conclusion

• “Big goals” – medical applications
• Self-regulated production of certain proteins – “preprogrammed assembly”