IGEM:Harvard/2007/Meetings/Week 9

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Stephanie's Powerpoint

Lunchtime meeting, 8/17

Harvard 2007 iGEM Jamboree Presentation Planning Meeting 1, 8/17

Content

Introduction

  • How cells physically interact with environment?
  • Underutilized aspects of iGEM: random libraries, surface expression
  • Figure of overall project: "Harvard iGEM 2007"; overall fusion of project


Targeting

  • Random library
  • Loop/terminal
  • Targets
    • Nickel
    • Calmodulin
    • EGF
    • GST
    • His
    • Strep
    • PDZ
  • Figures: Plates: before and after enrichment, somehow present numbers (pie chart? bar graph?), microscopy images with beads and fluorescent cells accumulating


Cell-cell signaling

  • QS – sender, receiver = biobricks, back to initial idea: target + QS, with figures
  • Figures: drop experiment (halo)



Intracellular signaling

  • Advantage of Fec system = gene expression with low level of background – need specificity with targets, etc, regulation on cell level rather than on population level
  • Figures: 3d structure, where and why we chose to alter loop region



Conclusion

  • “Big goals” – medical applications
  • Self-regulated production of certain proteins – “preprogrammed assembly”
  • Constructs / futures



SLIDE allotment

  • allow 45 seconds or so per slide
  • total of 15 ish slides
  • Intro = 2 slides
    • Slide 1: Why do we want to do this?
    • Slide 2: This is how we're going to do it; architecture of project
  • Targeting = 4
  • Cell-cell (QS) = 4
  • Intracellular signaling = 3
  • Conclusion = 2

TIME allotment

  • Intro: 1 min
  • Targeting: 3 min
  • Cell-cell signaling: 2.5 min
  • Intracellular signaling: 2.5 min
  • Conclusion: 1 min
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