IGEM:Harvard/2007/Plates

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(New page: Colony Count of Overnight Ligation vs. Shorter (2 hr Ligation) Plated 6-20-07 {| {{table}} | align="center" style="background:#f0f0f0;"|'''Cell Type''' | align="center" style="background:#...)
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| align="center" style="background:#f0f0f0;"|'''Colony Count <br>(Overnight Ligation)'''
| align="center" style="background:#f0f0f0;"|'''Colony Count <br>(Overnight Ligation)'''
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| OmpA1 + random library, PCR||275 µL||41||5
+
| OmpA1 + random library, PCR||275 µL||5||41
|-
|-
-
| OmpA1 + random library, PCR||25 µL||2||0
+
| OmpA1 + random library, PCR||25 µL||0||2
|-
|-
-
| OmpA1 + random library, extension||275 µL||30||0
+
| OmpA1 + random library, extension||275 µL||0||30
|-
|-
| OmpA1 + random library, extension||25 µL||0||0
| OmpA1 + random library, extension||25 µL||0||0
|-
|-
-
| OmpA2 + random library, PCR||275 µL||1||0
+
| OmpA2 + random library, PCR||275 µL||0||1
|-
|-
| OmpA2 + random library, PCR||25 µL||0||0
| OmpA2 + random library, PCR||25 µL||0||0
|-
|-
-
| OmpA2 + random library, extension||275 µL||77||12
+
| OmpA2 + random library, extension||275 µL||12||77
|-
|-
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| OmpA2 + random library, extension||25 µL||1||0
+
| OmpA2 + random library, extension||25 µL||0||1
|}
|}

Revision as of 14:25, 22 June 2007

Colony Count of Overnight Ligation vs. Shorter (2 hr Ligation) Plated 6-20-07

Cell Type Volume Plated Colony Count
(Short Ligation)
Colony Count
(Overnight Ligation)
OmpA1 + random library, PCR275 µL541
OmpA1 + random library, PCR25 µL02
OmpA1 + random library, extension275 µL030
OmpA1 + random library, extension25 µL00
OmpA2 + random library, PCR275 µL01
OmpA2 + random library, PCR25 µL00
OmpA2 + random library, extension275 µL1277
OmpA2 + random library, extension25 µL01
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