IGEM:Harvard/2007/Plates

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
Line 6: Line 6:
| align="center" style="background:#f0f0f0;"|'''Colony Count <br>(Short Ligation)'''
| align="center" style="background:#f0f0f0;"|'''Colony Count <br>(Short Ligation)'''
| align="center" style="background:#f0f0f0;"|'''Colony Count <br>(Overnight Ligation)'''
| align="center" style="background:#f0f0f0;"|'''Colony Count <br>(Overnight Ligation)'''
 +
| align="center" style="background:#f0f0f0;"|'''Colony Count <br>(Overweekend Ligation;1uL DNA)'''
 +
| align="center" style="background:#f0f0f0;"|'''Colony Count <br>(Overweekend Ligation;6uL DNA)'''
|-
|-
| OmpA1 + random library, PCR||275 µL||5||41
| OmpA1 + random library, PCR||275 µL||5||41

Revision as of 12:16, 26 June 2007

Colony Count of Overnight Ligation vs. Shorter (2 hr Ligation) Plated 6-20-07

Cell Type Volume Plated Colony Count
(Short Ligation)
Colony Count
(Overnight Ligation)
Colony Count
(Overweekend Ligation;1uL DNA)
Colony Count
(Overweekend Ligation;6uL DNA)
OmpA1 + random library, PCR275 µL541
OmpA1 + random library, PCR25 µL02
OmpA1 + random library, extension275 µL030
OmpA1 + random library, extension25 µL00
OmpA2 + random library, PCR275 µL01
OmpA2 + random library, PCR25 µL00
OmpA2 + random library, extension275 µL1277
OmpA2 + random library, extension25 µL01
Personal tools