IGEM:Harvard/2007/Plates

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| OmpA1 + random library, PCR||275 µL||5||41
| OmpA1 + random library, PCR||275 µL||5||41
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| OmpA1 + random library, PCR||25 µL||0||2
+
| OmpA1 + random library, PCR||25 µL||0||2||n/a||n/a
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| OmpA1 + random library, extension||275 µL||0||30
| OmpA1 + random library, extension||275 µL||0||30
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|-
-
| OmpA1 + random library, extension||25 µL||0||0
+
| OmpA1 + random library, extension||25 µL||0||0||n/a||n/a
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| OmpA2 + random library, PCR||275 µL||0||1
| OmpA2 + random library, PCR||275 µL||0||1
|-
|-
-
| OmpA2 + random library, PCR||25 µL||0||0
+
| OmpA2 + random library, PCR||25 µL||0||0||n/a||n/a
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| OmpA2 + random library, extension||275 µL||12||77
| OmpA2 + random library, extension||275 µL||12||77
|-
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| OmpA2 + random library, extension||25 µL||0||1
+
| OmpA2 + random library, extension||25 µL||0||1||n/a||n/a
|}
|}

Revision as of 12:17, 26 June 2007

Colony Count of Overnight Ligation vs. Shorter (2 hr Ligation) Plated 6-20-07

Cell Type Volume Plated Colony Count
(Short Ligation)
Colony Count
(Overnight Ligation)
Colony Count
(Overweekend Ligation;1uL DNA)
Colony Count
(Overweekend Ligation;6uL DNA)
OmpA1 + random library, PCR275 µL541
OmpA1 + random library, PCR25 µL02n/an/a
OmpA1 + random library, extension275 µL030
OmpA1 + random library, extension25 µL00n/an/a
OmpA2 + random library, PCR275 µL01
OmpA2 + random library, PCR25 µL00n/an/a
OmpA2 + random library, extension275 µL1277
OmpA2 + random library, extension25 µL01n/an/a
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