IGEM:Harvard/2007/Plates

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| align="center" style="background:#f0f0f0;"|'''Colony Count <br>(Short Ligation)'''
| align="center" style="background:#f0f0f0;"|'''Colony Count <br>(Short Ligation)'''
| align="center" style="background:#f0f0f0;"|'''Colony Count <br>(Overnight Ligation)'''
| align="center" style="background:#f0f0f0;"|'''Colony Count <br>(Overnight Ligation)'''
-
| align="center" style="background:#f0f0f0;"|'''Colony Count <br>(Overweekend Ligation;1uL DNA)'''
+
 
-
| align="center" style="background:#f0f0f0;"|'''Colony Count <br>(Overweekend Ligation;6uL DNA)'''
+
|-
|-
-
| OmpA1 + random library, PCR||275 µL||5||41||10||119
+
| OmpA1 + random library, PCR||275 µL||5||41
|-
|-
-
| OmpA1 + random library, PCR||25 µL||0||2||n/a||n/a
+
| OmpA1 + random library, PCR||25 µL||0||2
|-
|-
-
| OmpA1 + random library, extension||275 µL||0||30||0||127
+
| OmpA1 + random library, extension||275 µL||0||30
|-
|-
-
| OmpA1 + random library, extension||25 µL||0||0||n/a||n/a
+
| OmpA1 + random library, extension||25 µL||0||0
|-
|-
| OmpA2 + random library, PCR||275 µL||0||1
| OmpA2 + random library, PCR||275 µL||0||1
|-
|-
-
| OmpA2 + random library, PCR||25 µL||0||0||n/a||n/a
+
| OmpA2 + random library, PCR||25 µL||0||0
|-
|-
| OmpA2 + random library, extension||275 µL||12||77
| OmpA2 + random library, extension||275 µL||12||77
|-
|-
-
| OmpA2 + random library, extension||25 µL||0||1||n/a||n/a
+
| OmpA2 + random library, extension||25 µL||0||1
|}
|}
 +
 +
<br><br>
 +
{|  {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''Colony Count <br>(Overweekend Ligation;1uL DNA)'''
 +
| align="center" style="background:#f0f0f0;"|'''Colony Count <br>(Overweekend Ligation;6uL DNA)'''

Revision as of 12:24, 26 June 2007

Colony Count of Overnight Ligation vs. Shorter (2 hr Ligation) Plated 6-20-07

Cell Type Volume Plated Colony Count
(Short Ligation)
Colony Count
(Overnight Ligation)
OmpA1 + random library, PCR275 µL541
OmpA1 + random library, PCR25 µL02
OmpA1 + random library, extension275 µL030
OmpA1 + random library, extension25 µL00
OmpA2 + random library, PCR275 µL01
OmpA2 + random library, PCR25 µL00
OmpA2 + random library, extension275 µL1277
OmpA2 + random library, extension25 µL01



Colony Count
(Overweekend Ligation;1uL DNA)
Colony Count
(Overweekend Ligation;6uL DNA)
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