IGEM:Harvard/2007/Plates: Difference between revisions
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| align="center" style="background:#f0f0f0;"|'''Colony Count <br>(Short Ligation)''' | | align="center" style="background:#f0f0f0;"|'''Colony Count <br>(Short Ligation)''' | ||
| align="center" style="background:#f0f0f0;"|'''Colony Count <br>(Overnight Ligation)''' | | align="center" style="background:#f0f0f0;"|'''Colony Count <br>(Overnight Ligation)''' | ||
|- | |- | ||
| OmpA1 + random library, PCR||275 µL||5||41 | | OmpA1 + random library, PCR||275 µL||5||41 | ||
|- | |- | ||
| OmpA1 + random library, PCR||25 µL||0||2 | | OmpA1 + random library, PCR||25 µL||0||2 | ||
|- | |- | ||
| OmpA1 + random library, extension||275 µL||0||30 | | OmpA1 + random library, extension||275 µL||0||30 | ||
|- | |- | ||
| OmpA1 + random library, extension||25 µL||0||0 | | OmpA1 + random library, extension||25 µL||0||0 | ||
|- | |- | ||
| OmpA2 + random library, PCR||275 µL||0||1 | | OmpA2 + random library, PCR||275 µL||0||1 | ||
|- | |- | ||
| OmpA2 + random library, PCR||25 µL||0||0 | | OmpA2 + random library, PCR||25 µL||0||0 | ||
|- | |- | ||
| OmpA2 + random library, extension||275 µL||12||77 | | OmpA2 + random library, extension||275 µL||12||77 | ||
|- | |- | ||
| OmpA2 + random library, extension||25 µL||0||1 | | OmpA2 + random library, extension||25 µL||0||1 | ||
|} | |} | ||
<br><br> | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Colony Count <br>(Overweekend Ligation;1uL DNA)''' | |||
| align="center" style="background:#f0f0f0;"|'''Colony Count <br>(Overweekend Ligation;6uL DNA)''' | |||
Revision as of 09:24, 26 June 2007
Colony Count of Overnight Ligation vs. Shorter (2 hr Ligation) Plated 6-20-07
| Cell Type | Volume Plated | Colony Count (Short Ligation) |
Colony Count (Overnight Ligation) |
| OmpA1 + random library, PCR | 275 µL | 5 | 41 |
| OmpA1 + random library, PCR | 25 µL | 0 | 2 |
| OmpA1 + random library, extension | 275 µL | 0 | 30 |
| OmpA1 + random library, extension | 25 µL | 0 | 0 |
| OmpA2 + random library, PCR | 275 µL | 0 | 1 |
| OmpA2 + random library, PCR | 25 µL | 0 | 0 |
| OmpA2 + random library, extension | 275 µL | 12 | 77 |
| OmpA2 + random library, extension | 25 µL | 0 | 1 |
| Colony Count (Overweekend Ligation;1uL DNA) |
Colony Count (Overweekend Ligation;6uL DNA) |
