IGEM:Harvard/2007/Plates: Difference between revisions
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| align="center" style="background:#f0f0f0;"|'''0ng DNA''' | | align="center" style="background:#f0f0f0;"|'''0ng DNA''' | ||
| align="center" style="background:#f0f0f0;"|'''25ng DNA''' | | align="center" style="background:#f0f0f0;"|'''25ng DNA''' | ||
| align="center" style="background:# | | align="center" style="background:#ff0000;"|'''50ng DNA''' | ||
| align="center" style="background:#f0f0f0;"|'''100ng DNA''' | | align="center" style="background:#f0f0f0;"|'''100ng DNA''' | ||
| align="center" style="background:#f0f0f0;"|'''250ng DNA''' | | align="center" style="background:#f0f0f0;"|'''250ng DNA''' | ||
Revision as of 09:06, 12 July 2007
Colony Count of Overnight Ligation vs. Shorter (2 hr Ligation)
Plated 6-20-07
| Cell Type | Volume Plated | Colony Count (Short Ligation) |
Colony Count (Overnight Ligation) |
| OmpA1 + random library, PCR | 275 µL | 5 | 41 |
| OmpA1 + random library, PCR | 25 µL | 0 | 2 |
| OmpA1 + random library, extension | 275 µL | 0 | 30 |
| OmpA1 + random library, extension | 25 µL | 0 | 0 |
| OmpA2 + random library, PCR | 275 µL | 0 | 1 |
| OmpA2 + random library, PCR | 25 µL | 0 | 0 |
| OmpA2 + random library, extension | 275 µL | 12 | 77 |
| OmpA2 + random library, extension | 25 µL | 0 | 1 |
Colony Count of Overweekend Ligation
Plated 6/25/07
The following were all done with 275 uL:
| Cell Type | Colony Count (Overweekend Ligation;1uL DNA) |
Colony Count (Overweekend Ligation;6uL DNA) |
| OmpA1/Group1/S | 0 | 127 |
| OmpA1/Group1/S2 | 8 | 24 |
| OmpA1/Group2/B | 10 | 119 |
| OmpA1/Group2/S2 | 4 | 79 |
Colony Count of Electroporation
Plated 7/11/07
| Plate | Dilution of cells | 0ng DNA | 25ng DNA | 50ng DNA | 100ng DNA | 250ng DNA | 500ng DNA |
| LB-KAN | 10^-2 | 521 | 136 | 197 | 361 | ||
| LB | 10^-3 | 489 | 329 | 576 | 267 | 130 | 338 |
