IGEM:Harvard/2007/Plates

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(MACS and FACS Cell Sort with AIDA Construct)
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| AIDA1 + his, (-) fraction, FACS||10 µL||0||12
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==MACS Cell Sort with AIDA Construct-All Induced==
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Plated 7-20-07
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Cell Type'''
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| align="center" style="background:#f0f0f0;"|'''Volume Plated'''
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| align="center" style="background:#f0f0f0;"|'''Colony Count <br>(white)'''
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| align="center" style="background:#f0f0f0;"|'''Colony Count <br>(red)'''
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| AIDA1 + strep, (+) fraction, MACS||120 µL||101||3
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| AIDA1 + strep, (+) fraction, MACS||25 µL||13||2
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| AIDA1 + his, (+) fraction, MACS||120 µL||29||22
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| AIDA1 + his, (+) fraction, MACS||25 µL||5||4

Revision as of 09:22, 7 August 2007

Contents

Colony Count of Overnight Ligation vs. Shorter (2 hr Ligation)

Plated 6-20-07

Cell Type Volume Plated Colony Count
(Short Ligation)
Colony Count
(Overnight Ligation)
OmpA1 + random library, PCR275 µL541
OmpA1 + random library, PCR25 µL02
OmpA1 + random library, extension275 µL030
OmpA1 + random library, extension25 µL00
OmpA2 + random library, PCR275 µL01
OmpA2 + random library, PCR25 µL00
OmpA2 + random library, extension275 µL1277
OmpA2 + random library, extension25 µL01

Colony Count of Overweekend Ligation

Plated 6/25/07 The following were all done with 275 uL:

Cell Type Colony Count
(Overweekend Ligation;1uL DNA)
Colony Count
(Overweekend Ligation;6uL DNA)
OmpA1/Group1/S0127
OmpA1/Group1/S2824
OmpA1/Group2/B10119
OmpA1/Group2/S2479

Colony Count of Electroporation

Plated 7/11/07

Plate Dilution of cells 0ng DNA 25ng DNA 50ng DNA 100ng DNA 250ng DNA 500ng DNA
LB-KAN10^-2 521136197361
LB10^-3489329576267130338


MACS and FACS Cell Sort with AIDA Construct

Plated 7-20-07

Cell Type Volume Plated Colony Count
(white)
Colony Count
(red)
AIDA1 + strep, MACS5 µL50
AIDA1 + strep, MACS50 µL315
AIDA1 + strep, MACS300 µL23510
AIDA1 + his, MACS5 µL01
AIDA1 + his, MACS50 µL180
AIDA1 + his, MACS300 µL20611
Cell Type Volume Plated Colony Count
(white)
Colony Count
(red)
AIDA1 + strep, (+) fraction, FACS10 µL019
AIDA1 + strep, (-) fraction, FACS10 µL018
AIDA1 + his, (+) fraction, FACS10 µL18716
AIDA1 + his, (-) fraction, FACS10 µL012

MACS Cell Sort with AIDA Construct-All Induced

Plated 7-20-07

Cell Type Volume Plated Colony Count
(white)
Colony Count
(red)
AIDA1 + strep, (+) fraction, MACS120 µL1013
AIDA1 + strep, (+) fraction, MACS25 µL132
AIDA1 + his, (+) fraction, MACS120 µL2922
AIDA1 + his, (+) fraction, MACS25 µL54
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