IGEM:Harvard/2007/Plates: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 26: | Line 26: | ||
<br><br> | <br><br> | ||
<i>The following were all done with 275 uL:</i><br> | |||
{| {{table}} | {| {{table}} | ||
| align="center" style="background:#f0f0f0;"|'''Cell Type''' | |||
| align="center" style="background:#f0f0f0;"|'''Colony Count <br>(Overweekend Ligation;1uL DNA)''' | | align="center" style="background:#f0f0f0;"|'''Colony Count <br>(Overweekend Ligation;1uL DNA)''' | ||
| align="center" style="background:#f0f0f0;"|'''Colony Count <br>(Overweekend Ligation;6uL DNA)''' | | align="center" style="background:#f0f0f0;"|'''Colony Count <br>(Overweekend Ligation;6uL DNA)''' | ||
|- | |||
| OmpA1/Group1/S||0||127 | |||
|- | |||
| OmpA1/Group1/S2||8||24 | |||
|- | |||
| OmpA1/Group2/B||10||119 | |||
|- | |||
| OmpA1/Group2/S2||4||79 | |||
|} | |||
Revision as of 09:26, 26 June 2007
Colony Count of Overnight Ligation vs. Shorter (2 hr Ligation) Plated 6-20-07
| Cell Type | Volume Plated | Colony Count (Short Ligation) |
Colony Count (Overnight Ligation) |
| OmpA1 + random library, PCR | 275 µL | 5 | 41 |
| OmpA1 + random library, PCR | 25 µL | 0 | 2 |
| OmpA1 + random library, extension | 275 µL | 0 | 30 |
| OmpA1 + random library, extension | 25 µL | 0 | 0 |
| OmpA2 + random library, PCR | 275 µL | 0 | 1 |
| OmpA2 + random library, PCR | 25 µL | 0 | 0 |
| OmpA2 + random library, extension | 275 µL | 12 | 77 |
| OmpA2 + random library, extension | 25 µL | 0 | 1 |
The following were all done with 275 uL:
| Cell Type | Colony Count (Overweekend Ligation;1uL DNA) |
Colony Count (Overweekend Ligation;6uL DNA) |
| OmpA1/Group1/S | 0 | 127 |
| OmpA1/Group1/S2 | 8 | 24 |
| OmpA1/Group2/B | 10 | 119 |
| OmpA1/Group2/S2 | 4 | 79 |
