IGEM:Harvard/2007/Plates

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(MACS Cell Sort with AIDA Construct-All Induced)
Current revision (10:40, 13 August 2007) (view source)
(MACS Cell Sort with AIDA Sender Constructs)
 
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==MACS Cell Sort with AIDA Sender Constructs==
==MACS Cell Sort with AIDA Sender Constructs==
-
Plated 8-5-07
+
Plated 8-10-07
Note - Red colonies are what we want, white is background
Note - Red colonies are what we want, white is background
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|-
|-
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| AIDA1 + strep, (+) fraction, MACS||75 µL||325||24
+
| AIDA1 + strep sender, (+) fraction, MACS||75 µL||325||24
|-
|-
-
| AIDA1 + strep, (+) fraction, MACS||20 µL||103||8
+
| AIDA1 + strep sender, (+) fraction, MACS||20 µL||103||8
|-
|-
-
| AIDA1 + his, (+) fraction, MACS||75 µL||588||49
+
| AIDA1 + his sender, (+) fraction, MACS||75 µL||588||49
|-
|-
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| AIDA1 + his, (+) fraction, MACS||20 µL||317||30
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| AIDA1 + his sender, (+) fraction, MACS||20 µL||317||30
|}
|}

Current revision

Contents

Colony Count of Overnight Ligation vs. Shorter (2 hr Ligation)

Plated 6-20-07

Cell Type Volume Plated Colony Count
(Short Ligation)
Colony Count
(Overnight Ligation)
OmpA1 + random library, PCR275 µL541
OmpA1 + random library, PCR25 µL02
OmpA1 + random library, extension275 µL030
OmpA1 + random library, extension25 µL00
OmpA2 + random library, PCR275 µL01
OmpA2 + random library, PCR25 µL00
OmpA2 + random library, extension275 µL1277
OmpA2 + random library, extension25 µL01

Colony Count of Overweekend Ligation

Plated 6/25/07 The following were all done with 275 uL:

Cell Type Colony Count
(Overweekend Ligation;1uL DNA)
Colony Count
(Overweekend Ligation;6uL DNA)
OmpA1/Group1/S0127
OmpA1/Group1/S2824
OmpA1/Group2/B10119
OmpA1/Group2/S2479

Colony Count of Electroporation

Plated 7/11/07

Plate Dilution of cells 0ng DNA 25ng DNA 50ng DNA 100ng DNA 250ng DNA 500ng DNA
LB-KAN10^-2 521136197361
LB10^-3489329576267130338


MACS and FACS Cell Sort with AIDA Construct

Plated 7-20-07

Cell Type Volume Plated Colony Count
(white)
Colony Count
(red)
AIDA1 + strep, MACS5 µL50
AIDA1 + strep, MACS50 µL315
AIDA1 + strep, MACS300 µL23510
AIDA1 + his, MACS5 µL01
AIDA1 + his, MACS50 µL180
AIDA1 + his, MACS300 µL20611
Cell Type Volume Plated Colony Count
(white)
Colony Count
(red)
AIDA1 + strep, (+) fraction, FACS10 µL019
AIDA1 + strep, (-) fraction, FACS10 µL018
AIDA1 + his, (+) fraction, FACS10 µL18716
AIDA1 + his, (-) fraction, FACS10 µL012

MACS Cell Sort with AIDA Construct-All Induced

Plated 8-5-07

Cell Type Volume Plated Colony Count
(white)
Colony Count
(red)
AIDA1 + strep, (+) fraction, MACS120 µL1013
AIDA1 + strep, (+) fraction, MACS25 µL132
AIDA1 + his, (+) fraction, MACS120 µL2922
AIDA1 + his, (+) fraction, MACS25 µL54

MACS Cell Sort with AIDA Sender Constructs

Plated 8-10-07

Note - Red colonies are what we want, white is background

Cell Type Volume Plated Colony Count
(red)
Colony Count
(white)
AIDA1 + strep sender, (+) fraction, MACS75 µL32524
AIDA1 + strep sender, (+) fraction, MACS20 µL1038
AIDA1 + his sender, (+) fraction, MACS75 µL58849
AIDA1 + his sender, (+) fraction, MACS20 µL31730
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