IGEM:Harvard/2007/Plates

Colony Count of Overnight Ligation vs. Shorter (2 hr Ligation) Plated 6-20-07

Cell Type	Volume Plated	Colony Count (Short Ligation)	Colony Count (Overnight Ligation)
OmpA1 + random library, PCR	275 µL	5	41
OmpA1 + random library, PCR	25 µL	0	2
OmpA1 + random library, extension	275 µL	0	30
OmpA1 + random library, extension	25 µL	0	0
OmpA2 + random library, PCR	275 µL	0	1
OmpA2 + random library, PCR	25 µL	0	0
OmpA2 + random library, extension	275 µL	12	77
OmpA2 + random library, extension	25 µL	0	1

Colony Count (Overweekend Ligation;1uL DNA)

Colony Count (Overweekend Ligation;6uL DNA)