IGEM:Harvard/2007/Plates
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Colony Count of Overnight Ligation vs. Shorter (2 hr Ligation) Plated 6-20-07
Cell Type | Volume Plated | Colony Count (Short Ligation) |
Colony Count (Overnight Ligation) |
OmpA1 + random library, PCR | 275 µL | 41 | 5 |
OmpA1 + random library, PCR | 25 µL | 2 | 0 |
OmpA1 + random library, extension | 275 µL | 30 | 0 |
OmpA1 + random library, extension | 25 µL | 0 | 0 |
OmpA2 + random library, PCR | 275 µL | 1 | 0 |
OmpA2 + random library, PCR | 25 µL | 0 | 0 |
OmpA2 + random library, extension | 275 µL | 77 | 12 |
OmpA2 + random library, extension | 25 µL | 1 | 0 |