IGEM:Harvard/2007/Plates

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Colony Count of Overnight Ligation vs. Shorter (2 hr Ligation) Plated 6-20-07

Cell Type Volume Plated Colony Count
(Short Ligation)
Colony Count
(Overnight Ligation)
OmpA1 + random library, PCR275 µL415
OmpA1 + random library, PCR25 µL20
OmpA1 + random library, extension275 µL300
OmpA1 + random library, extension25 µL00
OmpA2 + random library, PCR275 µL10
OmpA2 + random library, PCR25 µL00
OmpA2 + random library, extension275 µL7712
OmpA2 + random library, extension25 µL10
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