IGEM:Harvard/2007/Plates

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Colony Count of Overnight Ligation vs. Shorter (2 hr Ligation) Plated 6-20-07

Cell Type Volume Plated Colony Count
(Short Ligation)
Colony Count
(Overnight Ligation)
OmpA1 + random library, PCR 275 µL 41 5
OmpA1 + random library, PCR 25 µL 2 0
OmpA1 + random library, extension 275 µL 30 0
OmpA1 + random library, extension 25 µL 0 0
OmpA2 + random library, PCR 275 µL 1 0
OmpA2 + random library, PCR 25 µL 0 0
OmpA2 + random library, extension 275 µL 77 12
OmpA2 + random library, extension 25 µL 1 0