IGEM:Harvard/2007/Plates

Colony Count of Overnight Ligation vs. Shorter (2 hr Ligation) Plated 6-20-07

Cell Type	Volume Plated	Colony Count (Short Ligation)	Colony Count (Overnight Ligation)
OmpA1 + random library, PCR	275 µL	5	41
OmpA1 + random library, PCR	25 µL	0	2
OmpA1 + random library, extension	275 µL	0	30
OmpA1 + random library, extension	25 µL	0	0
OmpA2 + random library, PCR	275 µL	0	1
OmpA2 + random library, PCR	25 µL	0	0
OmpA2 + random library, extension	275 µL	12	77
OmpA2 + random library, extension	25 µL	0	1

The following were all done with 275 uL:

Cell Type	Colony Count (Overweekend Ligation;1uL DNA)	Colony Count (Overweekend Ligation;6uL DNA)
OmpA1/Group1/S	0	127
OmpA1/Group1/S2	8	24
OmpA1/Group2/B	10	119
OmpA1/Group2/S2	4	79