IGEM:Harvard/2008/Lab Notebooks/DailyBook/Week1: Difference between revisions
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| align="center" style="background:#eeeeee;"|'''Size''' | | align="center" style="background:#eeeeee;"|'''Size''' | ||
| align="center" style="background:#eeeeee;"|'''Marker''' | | align="center" style="background:#eeeeee;"|'''Marker''' | ||
| align="center" style="background:#eeeeee;"| | | align="center" style="background:#eeeeee;"|'''Number of Colonies''' | ||
|- | |- | ||
| [http://partsregistry.org/Part:BBa_M30109 P11]||Light responsive system, dual regulation||pUC19-derived pMB1||4333||Amp/Cm | | [http://partsregistry.org/Part:BBa_M30109 P11]||Light responsive system, dual regulation||pUC19-derived pMB1||4333||Amp/Cm||224 | ||
|- | |- | ||
| [http://partsregistry.org/Part:BBa_I13522 P15]||GFP with Tet promoter||ColE1||937||Amp | | [http://partsregistry.org/Part:BBa_I13522 P15]||GFP with Tet promoter||ColE1||937||Amp||360 | ||
|- | |- | ||
| [http://partsregistry.org/Part:BBa_R0040 P16]||Tet repressible promoter||pMB1||54||Amp | | [http://partsregistry.org/Part:BBa_R0040 P16]||Tet repressible promoter||pMB1||54||Amp||264 | ||
|- | |- | ||
| [http://partsregistry.org/Part:BBa_Q04400 P17]||Inverter (TetR w/o promoter and Tet promoter)||OriS, P1 lytic, F1||902||Kan | | [http://partsregistry.org/Part:BBa_Q04400 P17]||Inverter (TetR w/o promoter and Tet promoter)||OriS, P1 lytic, F1||902||Kan||2 | ||
|- | |- | ||
| [http://partsregistry.org/Part:BBa_R0051 P18]||lambda promoter (cI regulated)||pMB1||49||Amp | | [http://partsregistry.org/Part:BBa_R0051 P18]||lambda promoter (cI regulated)||pMB1||49||Amp||112 | ||
|- | |- | ||
| [http://partsregistry.org/Part:BBa_I763007 P19]||RFP with lambda promoter||pMB1||918||Amp | | [http://partsregistry.org/Part:BBa_I763007 P19]||RFP with lambda promoter||pMB1||918||Amp||104 | ||
|- | |- | ||
| [http://partsregistry.org/Part:BBa_J04431 P20]||GFP (LVA tagged) with Lac promoter||pMB1||1122||Amp, Kan | | [http://partsregistry.org/Part:BBa_J04431 P20]||GFP (LVA tagged) with Lac promoter||pMB1||1122||Amp, Kan||136 | ||
|} | |} | ||
Revision as of 12:01, 23 June 2008
Monday: June 23, 2008
Chemical
Results from Friday (6/20) Electroporation and Transformation in S1
Name Registry Name Description Origin Marker Observations P1a J23113 Low promoter GFP tester pMB1 Kan 16 pink colonies of approx 7mm diameter P2a J23150 Medium promoter GFP tester pMB1 Kan no growth P3a J23151 High promoter GFP tester pMB1 Kan 8 pink colonies of approx 7mm diameter P12 pETDuet-1 Low copy plasmid pBR322-derived ColE1 Amp Lawn P13 pCDFDuet-1 Low copy plasmid CloDF13 Sm 41 pink colonies and some small yellow ones that may be E. coli P14 pCOLADuet-1 Low copy plasmid ColA Kan No growth
Light
Transformations of P11, P15-20
| Name | Description | Origin | Size | Marker | Number of Colonies |
| P11 | Light responsive system, dual regulation | pUC19-derived pMB1 | 4333 | Amp/Cm | 224 |
| P15 | GFP with Tet promoter | ColE1 | 937 | Amp | 360 |
| P16 | Tet repressible promoter | pMB1 | 54 | Amp | 264 |
| P17 | Inverter (TetR w/o promoter and Tet promoter) | OriS, P1 lytic, F1 | 902 | Kan | 2 |
| P18 | lambda promoter (cI regulated) | pMB1 | 49 | Amp | 112 |
| P19 | RFP with lambda promoter | pMB1 | 918 | Amp | 104 |
| P20 | GFP (LVA tagged) with Lac promoter | pMB1 | 1122 | Amp, Kan | 136 |
Restreaking
6/23: P16, which has GFP under a constitutive Tet promoter did not appear to fluoresce, so we restreaked all the of the plates (with the same antibiotic on which they were originally grown). They were left in the 37 degree incubator along with a blank Kan and a blank Amp plate (- controls).
Overnight cultures
6/23: The above was repeated in 5mL LB liquid cultures instead of plates.
Widgetry
Tuesday: June 24, 2008
Wednesday: June 25, 2008
Thursday: June 26, 2008
Friday: June 27, 2008
