IGEM:Harvard/2008/Lab Notebooks/DailyBook/Week1

/Chemical and Light/
/Widgetry/

Monday Meeting Notes

Goals for Week 1

Light team

• knockout protocol
• cloning part

Chemical team

• transform registry DNA into Shewie
• confirm Shewie growth via PCR

Widget team

• create basic MFC protocol
• speak with Pete and confirm design
• think about computer interfacing
  • LabView/MatLab?
  • Buy computer?

Team Goals

• anaerobic Shewie?
• manganese oxide?
• meet with Diane Newman

Things to ask for

Mutants from Orianna

• mtrA
• mtrB
• both

UT Austin

• OmpR knockout

Protocols

• Homologous recombination (Pacific Northwest?)
• Cre-Lox (Orianna)
• Colorimetric assay (Orianna)
• Shewie transformation (Diane Newman)

---