IGEM:Harvard/2008/Lab Notebooks/DailyBook/Week11/Chemical and Light: Difference between revisions
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Meng Xiao He (talk | contribs) (New page: =Retransformations 9/15= P126 and 127 retransformed into DH5α. Split 30μL and rest onto 2 diff CM plates. =Ligations 9/15= * Tried the Takara ligation kit: 1μL vector, 4μL insert, 5μL...) |
Meng Xiao He (talk | contribs) |
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==Lac QPI== | ==Lac QPI== | ||
P3+51 EX (8/3, dephos) with P38 oligo mix (Amy's prep) | P3+51 EX (8/3, dephos) with P38 oligo mix (Amy's prep) | ||
0 colonies (on KAN) | |||
Revision as of 06:20, 16 September 2008
Retransformations 9/15
P126 and 127 retransformed into DH5α. Split 30μL and rest onto 2 diff CM plates.
Ligations 9/15
- Tried the Takara ligation kit: 1μL vector, 4μL insert, 5μL ligation mix
- Transformed into DH5α
mtrB
P63 EX (8/14, dephos) with:
- mtrB TOPO ES (8/15)
- mtrB BB ES (8/14)
P97 SP (dephos) with:
- mtrB BB XP (8/14)
- mtrB TOPO XP (8/15)
Lac QPI
P3+51 EX (8/3, dephos) with P38 oligo mix (Amy's prep)
0 colonies (on KAN)
