IGEM:Harvard/2008/Lab Notebooks/DailyBook/Week11/Chemical and Light

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Revision as of 09:45, 15 September 2008 by Meng Xiao He (talk | contribs) (New page: =Retransformations 9/15= P126 and 127 retransformed into DH5α. Split 30μL and rest onto 2 diff CM plates. =Ligations 9/15= * Tried the Takara ligation kit: 1μL vector, 4μL insert, 5μL...)
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Retransformations 9/15

P126 and 127 retransformed into DH5α. Split 30μL and rest onto 2 diff CM plates.

Ligations 9/15

  • Tried the Takara ligation kit: 1μL vector, 4μL insert, 5μL ligation mix
  • Transformed into DH5α

mtrB

P63 EX (8/14, dephos) with:

  • mtrB TOPO ES (8/15)
  • mtrB BB ES (8/14)

P97 SP (dephos) with:

  • mtrB BB XP (8/14)
  • mtrB TOPO XP (8/15)

Lac QPI

P3+51 EX (8/3, dephos) with P38 oligo mix (Amy's prep)

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