IGEM:Harvard/2008/Lab Notebooks/DailyBook/Week12/Chemical and Light
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9/22 RE digests
With NEB enzymes, 60 min (buffer 2+BSA):
- 128#1 XPXmnI
- 128#5 XSXmnI
- 128#9 ESXmnI
The XmnI is to cut the backbone, which has a similar length to the mtrB constructs.
With Fermentas enzymes, 25 min:
- 116 SP
- 117 SP
- 108 SP
- 51 EX
- 63 XP
9/23 ligations
The following ligations were performed using 1:6 molar ratio and the Takara kit at 16°C for 45min:
