IGEM:Harvard/2008/Lab Notebooks/DailyBook/Week13/Chemical and Light

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(Amy's mutants)
(10-3 ligations)
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[[Image:2008-09-30_amymut.jpg]]
[[Image:2008-09-30_amymut.jpg]]
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=10-3 ligations=
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=10/03 Ligations/transformations=
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All transformed into XL1-blue.
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We ligated the following plasmids using volumetric ratios. The ligations were transformed into XL1-Blue.
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*108>131
*108>131
*38+51>131
*38+51>131

Revision as of 16:04, 3 October 2008

09/30 PCR

Should grow only on carb

  • 1-30: 20, 23, 25 (picked all three)
  • 31-60: 31-60 (picked 31, 35, 39, 42, 46, 50, 55, 60)

Should grow only on kan

  • 61-90: none
  • 91-120: none
  • 121-150: none

--Upload picture of colony PCR gel--

Picked colony 20 (P38+130) and 31, 39, 60 (P39+130) and grew them up in liquid cultures (LB Amp).

Amy's mutants

Image:2008-09-30_amymut.jpg

10/03 Ligations/transformations

We ligated the following plasmids using volumetric ratios. The ligations were transformed into XL1-Blue.

  • 108>131
  • 38+51>131
  • 122>131
  • 38+130>101
  • 130>133
  • 130>134
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