IGEM:Harvard/2008/Lab Notebooks/DailyBook/Week13/Chemical and Light: Difference between revisions
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[[Image:2008-09-30_amymut.jpg]] | [[Image:2008-09-30_amymut.jpg]] | ||
=10/03 | =Ligations/transformations 10/03 = | ||
We ligated the following plasmids using volumetric ratios. The ligations were transformed into XL1-Blue. | We ligated the following plasmids using volumetric ratios. The ligations were transformed into XL1-Blue. | ||
*108>131 | *108>131 | ||
Revision as of 14:04, 3 October 2008
09/30 PCR
Should grow only on carb
- 1-30: 20, 23, 25 (picked all three)
- 31-60: 31-60 (picked 31, 35, 39, 42, 46, 50, 55, 60)
Should grow only on kan
- 61-90: none
- 91-120: none
- 121-150: none
--Upload picture of colony PCR gel--
Picked colony 20 (P38+130) and 31, 39, 60 (P39+130) and grew them up in liquid cultures (LB Amp).
Amy's mutants
Ligations/transformations 10/03
We ligated the following plasmids using volumetric ratios. The ligations were transformed into XL1-Blue.
- 108>131
- 38+51>131
- 122>131
- 38+130>101
- 130>133
- 130>134
