IGEM:Harvard/2008/Lab Notebooks/DailyBook/Week13/Chemical and Light: Difference between revisions
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=09/30 PCR= | =09/30 PCR= | ||
Should grow only on carb | Should grow only on carb | ||
*1-30: 20, 23, 25 (picked all three) | *1-30: 20, 23, 25 (picked all three) --> P38+130 | ||
*31-60: 31-60 (picked 31, 35, 39, 42, 46, 50, 55, 60) | *31-60: 31-60 (picked 31, 35, 39, 42, 46, 50, 55, 60) --> P39+130 | ||
Should grow only on kan | Should grow only on kan | ||
*61-90: none | *61-90: none --> P116+130 | ||
*91-120: none | *91-120: none --> P117+130 | ||
*121-150: none | *121-150: none --> P108+130 | ||
--Upload picture of colony PCR gel-- | --Upload picture of colony PCR gel-- | ||
Picked colony 20 (P38+130) and 31, 39, 60 (P39+130) and grew them up in liquid cultures (LB Amp). | Picked colony 20 (P38+130) and 31, 39, 60 (P39+130) and grew them up in liquid cultures (LB Amp). | ||
=Amy's mutants= | =Amy's mutants= | ||
[[Image:2008-09-30_amymut.jpg]] | [[Image:2008-09-30_amymut.jpg]] | ||
Revision as of 07:18, 4 October 2008
09/30 PCR
Should grow only on carb
- 1-30: 20, 23, 25 (picked all three) --> P38+130
- 31-60: 31-60 (picked 31, 35, 39, 42, 46, 50, 55, 60) --> P39+130
Should grow only on kan
- 61-90: none --> P116+130
- 91-120: none --> P117+130
- 121-150: none --> P108+130
--Upload picture of colony PCR gel--
Picked colony 20 (P38+130) and 31, 39, 60 (P39+130) and grew them up in liquid cultures (LB Amp).
Amy's mutants
Ligations/transformations 10/03
We ligated the following plasmids. The ligations were transformed into XL1-Blue.
- 108>131
- 38+51>131
- 122>131
- 38+130>101
- 130>133
- 130>134
Shewanella
We ethanol precipitated the above ligations in preparation for electroporation of Shewanella.
