IGEM:Harvard/2008/Lab Notebooks/DailyBook/Week13/Chemical and Light

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(Ligations/transformations 10/03)
(09/30 PCR)
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=09/30 PCR=
=09/30 PCR=
Should grow only on carb
Should grow only on carb
-
*1-30: 20, 23, 25 (picked all three)
+
*1-30: 20, 23, 25 (picked all three) --> P38+130
-
*31-60: 31-60 (picked 31, 35, 39, 42, 46, 50, 55, 60)
+
*31-60: 31-60 (picked 31, 35, 39, 42, 46, 50, 55, 60) --> P39+130
Should grow only on kan
Should grow only on kan
-
*61-90: none
+
*61-90: none --> P116+130
-
*91-120: none
+
*91-120: none --> P117+130
-
*121-150: none
+
*121-150: none --> P108+130
--Upload picture of colony PCR gel--
--Upload picture of colony PCR gel--
Picked colony 20 (P38+130) and 31, 39, 60 (P39+130) and grew them up in liquid cultures (LB Amp).
Picked colony 20 (P38+130) and 31, 39, 60 (P39+130) and grew them up in liquid cultures (LB Amp).
 +
=Amy's mutants=
=Amy's mutants=
[[Image:2008-09-30_amymut.jpg]]
[[Image:2008-09-30_amymut.jpg]]

Revision as of 09:18, 4 October 2008

Contents

09/30 PCR

Should grow only on carb

  • 1-30: 20, 23, 25 (picked all three) --> P38+130
  • 31-60: 31-60 (picked 31, 35, 39, 42, 46, 50, 55, 60) --> P39+130

Should grow only on kan

  • 61-90: none --> P116+130
  • 91-120: none --> P117+130
  • 121-150: none --> P108+130

--Upload picture of colony PCR gel--

Picked colony 20 (P38+130) and 31, 39, 60 (P39+130) and grew them up in liquid cultures (LB Amp).

Amy's mutants

Image:2008-09-30_amymut.jpg

Ligations/transformations 10/03

We ligated the following plasmids. The ligations were transformed into XL1-Blue.

  • 108>131
  • 38+51>131
  • 122>131
  • 38+130>101
  • 130>133
  • 130>134

Shewanella

We ethanol precipitated the above ligations in preparation for electroporation of Shewanella.

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